Addgene

We narrowed to 1,126 results for: fret

Showing: 181 - 200 of 1126 results

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pDRF1-GW yAT1.03R122KR126K

Plasmid

#132782

Purpose

Non-binding version of the FRET biosensor for ATP measurements in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

Insert

yAT1.03R122KR126K ymTq2-tdTomato

Use

Tags

Expression

Yeast

Mutation

R122K, R126K

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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MCS Lck only

Plasmid

#90411

Purpose

A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.

Depositor

Katharina Gaus

Article

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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GW1-mScarlet-Epac-GeNL

Plasmid

#207874

Purpose

Mammalian expression of the FRET/BRET cAMP sensor

Depositor

Mathew Tantama

Article

French et al ACS Omega. 2019 Sep 12;4(13):15504-15511

Insert

mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-ICUE2

Plasmid

#173017

Purpose

Improved green-red FRET based cAMP indicator.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-ICUE2

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2

Plasmid

#99868

Purpose

Measure FRET between LSSmOrange and mKate2

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

LSSmOrange

Use

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Article

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

A206K both

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mScarletI-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Mito-Lapronic/pGW1

Plasmid

#140754

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Mito-Lapronic

Use

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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Ateam1.03-nD/nA/pENTR1A

Plasmid

#51959

Purpose

Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
Ateam1.03-nD/nA/pRSETB

Plasmid

#51960

Purpose

bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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mKOkappa-2A-mTurquoise2

Plasmid

#98837

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKOkappa-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mKO2-2A-mTurquoise2

Plasmid

#98838

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKO2-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Lapronic/pRSETB

Plasmid

#140755

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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sReach-mTurquoise2

Plasmid

#98840

Purpose

Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

sReach-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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