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We narrowed to 679 results for: intein

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Showing: 181 - 200 of 679 results

  14. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  15. pMAL-c2X_MBP-LcgcN(C24S)-H6

    Plasmid
    #245527
    Purpose
    Maltose binding protein N-terminally fused to His-tagged N-terminal LCGC14 PolB2split intein fragment
    Depositor
    Henning Mootz
    Article
    Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202
    Insert
    N-terminal Fragment of the LCGC14 Split Intein with C24S Mutation
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    C24S within the N-terminal fragment of the LCGC14…
    Promoter
    tac
    Available Since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    245527-plasmid-map-sequence-id-492806

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  19. pMAL-c2X_MBP-CLmN-H6

    Plasmid
    #234487
    Purpose
    Maltose binding protein N-terminally fused to His-tagged CLmN split intein
    Depositor
    Henning Mootz
    Article
    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:
    Insert
    N-terminal Fragment of the CLm Split Intein
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    T69K, F75H, M118N within the N-terminal fragment …
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234487-plasmid-map-sequence-id-471039

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  20. p3x-Flag Gli3 37R3-2

    Plasmid
    #120305
    Purpose
    Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C
    Depositor
    David Liu
    Article
    Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi
    Insert
    Intein 37R3-2, Gli3
    Use
    Synthetic Biology
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Contains mutations as reported in 2011 Chemistry …
    Promoter
    CMV
    Available Since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    120305-plasmid-map-sequence-id-234346

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Showing: 181 - 200 of 679 results