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We narrowed to 527 results for: plasmid cloning by pcr

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Showing: 181 - 200 of 527 results

  5. pATF1-donor

    Plasmid
    #112328
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF1 with GFP
    Depositor
    Kevin White
    Insert
    ATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
    Use
    CRISPR
    Mutation
    hg38:12:50819876:T>A, hg38:12:50819888:T>C,…
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112328-plasmid-map-sequence-id-228551

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  6. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Phillip Zamore
    Insert
    aubergine (aub Fly)
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available Since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69448-plasmid-map-sequence-id-122550

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  7. GLRX3

    Plasmid
    #39036
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX3 (GLRX3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    A PCR artifact adds a C-terminal sequence includi…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. LHP1034 - ECFP-Nterm

    Plasmid
    #32436
    Depositor
    Linda Hicke
    Insert
    ECFP
    Use
    Cloning
    Available Since
    Oct. 21, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. LHP1032 - EYFP-Nterm

    Plasmid
    #32438
    Depositor
    Linda Hicke
    Insert
    EYFP
    Use
    Cloning
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. LHP1033 - EYFP-Cterm

    Plasmid
    #32437
    Depositor
    Linda Hicke
    Insert
    EYFP
    Use
    Cloning
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. LHP1035 - ECFP-Cterm

    Plasmid
    #32435
    Depositor
    Linda Hicke
    Insert
    ECFP
    Use
    Cloning
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. pHMR1B

    Plasmid
    #102958
    Purpose
    gene cloning of MR1B
    Depositor
    Gennaro De Libero, Lucia Mori
    Insert
    MR1B cDNA (MR1P1 Human)
    Use
    Cloning
    Mutation
    Stop codon has been removed by HindIII site
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102958-plasmid-map-sequence-id-198778

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  17. PB-XL-AncBE4max

    Plasmid
    #136254
    Purpose
    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
    Depositor
    Zhaohui Ye
    Article
    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:
    Insert
    AncBE4max_GFP
    Use
    CRISPR; Piggybac dna transposon
    Expression
    Mammalian
    Mutation
    The AncBE4max_GFP insert was PCR amplified from a…
    Promoter
    TRE3G
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136254-plasmid-map-sequence-id-270570

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Showing: 181 - 200 of 527 results