We narrowed to 2,466 results for: crispr system

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pBT009.J3.RFP

Plasmid

#177324

Purpose

Activatable RFP expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Insert

mRFP1

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK050

Plasmid

#65157

Purpose

Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Type

Empty backbone

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-KRAB

Plasmid

#191314

Purpose

piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

KRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pX330_human CDK1

Plasmid

#118597

Purpose

One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

guide RNA against human CDK1

Use

CRISPR

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

Plasmid

#92099

Purpose

Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Expression

Mammalian

Promoter

Tet-On 3G Bidirectional

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-tTRE-scFv-SunTag

Plasmid

#193138

Purpose

Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

scFv-SunTag

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(AtUBI10)

Plasmid

#202016

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

AtUBI10

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Csy4-PE2

Plasmid

#172667

Purpose

Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

Csy4-PE2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Expression

Mammalian

Promoter

Ef1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(CmYLCV)

Plasmid

#202014

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

CmYLCV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtUBQ10 (GB2478)

Plasmid

#160594

Purpose

UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtUBQ10

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

Plasmid

#140228

Purpose

RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1 (Cas12a) N terminal half (406) with zipper

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

RRM2 endogenous promoter

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_VEGFA-mcherry

Plasmid

#159790

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human VEGFA gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting VEGFA gene (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

Plasmid

#140227

Purpose

Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR

Use

Lentiviral

Expression

Mammalian

Promoter

HRE (Hypoxia inducible)

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_HOXD13-mcherry

Plasmid

#159793

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting HOXD13 gene (HOXD13 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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