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EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdMCP Halo

Plasmid

#104098

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdMCP-Halo

Use

Lentiviral

Tags

C-terminal Halo-tag, N-terminal NLS, and Two syno…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Mito-LOV-Turbo-V5

Plasmid

#199666

Purpose

expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

COX4I1 mito targeting sequence and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA3_P2A_Hygro_Barcode

Plasmid

#120440

Purpose

Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA3 (FOXA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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phage UbiC SNAP-DDX6

Plasmid

#119948

Purpose

Lentiviral vector expressing SNAP-DDX6 fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

SNAP

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-IGFBP2

Plasmid

#191006

Purpose

Lentiviral expression vector for IGFBP2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA1_P2A_Hygro_Barcode

Plasmid

#120438

Purpose

Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_hNgn1_UBC_Bla

Plasmid

#61473

Purpose

3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn1 (NEUROG1 Human)
Bla

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-RFC1

Plasmid

#59209

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRC10-EFS-huLbCpf1-2A-Blast-WPRE

Plasmid

#123359

Purpose

Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM2

Plasmid

#82537

Purpose

Lentiviral vector for expressing HRP-LRRTM2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM2 (LRRTM2 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAH1 (lenti hLPL-FLAG)

Plasmid

#102448

Purpose

FLAG-tagged human LPL in lentiviral vector

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

Lipoprotein lipase (LPL Human)

Use

Lentiviral

Tags

6x His and FLAG

Expression

Mutation

Promoter

CMV

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

Plasmid

#139448

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC_P2A_Hygro_Barcode

Plasmid

#120461

Purpose

Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM1

Plasmid

#82536

Purpose

Lentiviral vector for expressing HRP-LRRTM1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM1 (LRRTM1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P202A/F203A)-FLAG

Plasmid

#164976

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P202A/ F203A

Promoter

EF1a

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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