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pSFS2A-CaHygB

Plasmid

#189564

Purpose

CaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans

Depositor

Richard Bennett

Article

Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

Insert

HygB

Expression

Yeast

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Gi1-CASE

Plasmid

#168120

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at A121/E122 within GNAI1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

Plasmid

#233080

Purpose

Expression vector for sgRNA-S1b and eCas12f1i

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.3 Thy1.1

Plasmid

#14749

Depositor

Christophe Benoist, Diane Mathis

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Expression

Yeast

Promoter

GPM1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Expression

Yeast

Promoter

FBA1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

Plasmid

#89693

Purpose

Luciferase reporter plasmid targeted at csr-1 locus

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gsy-1 promoter, luciferase

Use

Yeast episomal vector

Tags

luciferase

Promoter

gsy-1

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gαi*-BERKY3

Plasmid

#158424

Purpose

BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Use

Lentiviral

Tags

myc

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA6-mRuby3

Plasmid

#184892

Purpose

Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA6 (ANXA6 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R587K

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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