We narrowed to 8,863 results for: BLI;

1. pGB-06-07-Vps34

   Plasmid

   #187989

   Purpose

   Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323

   Depositor

   Sascha Martens

   Insert

   Vps34 (PIK3C3 Human)
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2151 pHAGE-hSPC-TFEB-TagBFP-W

   Plasmid

   #188722

   Purpose

   Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein

   Depositor

   Darrell Kotton

   Insert

   TFEB (TFEB Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mutation

   Promoter

   hSP-C

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCW57.1-GFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132622

   Purpose

   Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mT2A-TagRFP-T-linker-dre-PBD*-FNF

   Plasmid

   #182342

   Purpose

   Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-TagRFP-T-linker-dre-PBD*-FNF
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGT270

   Plasmid

   #171878

   Purpose

   Start-Stop Level 2 destination vector for Synechocystis sp. PCC 6803

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pStA0::ECK120015170

   Plasmid

   #171879

   Purpose

   Terminator ECK120015170 in pStA0

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

   Insert

   Terminator ECK120015170
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CNN3A-c007

   Plasmid

   #175457

   Purpose

   Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.

   Depositor

   Opher Gileadi

   Insert

   CNN3 (CNN3 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV V5-Eva1b

   Plasmid

   #175158

   Purpose

   Lentiviral expression of V5-tagged mouse Eva1b

   Depositor

   Larry Gerace

   Insert

   Eva1b (Eva1b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV Cenpv-V5

    Plasmid

    #175125

    Purpose

    Lentiviral expression of mouse Cenpv-V5

    Depositor

    Larry Gerace

    Insert

    Cenpv (Cenpv Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330.orup

    Plasmid

    #110404

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Clim Act MCH (CAM)

    Plasmid

    #104443

    Purpose

    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Inaki Ruiz-Trillo, Elena Casacuberta

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    actin

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Clim EF1 MCH (CEM)

    Plasmid

    #104444

    Purpose

    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Inaki Ruiz-Trillo, Elena Casacuberta

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Clim Tub MCH (CTM)

    Plasmid

    #104445

    Purpose

    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Inaki Ruiz-Trillo, Elena Casacuberta

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    tubulin promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lentiviral human Citron shRNA 1 GFP

    Plasmid

    #155286

    Purpose

    Lentiviral expression of human CIT shRNA, GFP expression, based on Addgene 12247

    Depositor

    Martin Carroll

    Insert

    Citron (CIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-hSPC-TFEB(delta30)eGFP-W

    Plasmid

    #126693

    Purpose

    Lineage specific over expression of a mutated TFEB in lung alveolar epithelial cells, where TFEB is missing the first 30 amino acids of the sequence.

    Depositor

    Darrell Kotton

    Insert

    TFEB(delta30) (TFEB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted amino acids 1-30, H32D, Deleted Stop Codo…

    Promoter

    hSPC

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLL1273

    Plasmid

    #129288

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Galp_687 as inserts

    Depositor

    Lee Lynd

    Inserts

    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-Galp_687 (Ga0256695_ 0687)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLL1274

    Plasmid

    #129385

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_996 as inserts

    Depositor

    Lee Lynd

    Inserts

    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-AraF_996 (Ga0256695_ 0996)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc var1 K51/52 to NQ

    Plasmid

    #127287

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K51 and 52 to N and Q by Q5 mutagenesis

    Promoter

    CMV

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ELL3 Streptag CtoA

    Plasmid

    #127283

    Purpose

    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity

    Depositor

    Christine Milcarek

    Insert

    ELL3 (NM_145973) (ELL3 Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHQK

    Promoter

    EF1a

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ELL1 Streptag

    Plasmid

    #127278

    Purpose

    Expresses mouse ELL1 with streptactin tag in mammalian cells

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924) (ELL Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Myc var1 K397R

    Plasmid

    #127285

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K397R by Q5 mutagenesis

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Myc var1

    Plasmid

    #127284

    Purpose

    Expresses Myc to see if degraded by ELLs

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    transcript variant 1

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Myc var 1 K396/397 to NQ

    Plasmid

    #127286

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K396 and 397 to N and Q by Q5 mutagenesi

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHH0103 UTROPHIN WW domain

    Plasmid

    #104275

    Purpose

    Bacterial expression of WW domain from UTROPHIN

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    UTROPHIN WW domain (UTRN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103 KIAA1688 WW domain #1

    Plasmid

    #104277

    Purpose

    Bacterial expression of WW domain #1 from KIAA1688

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    KIAA1688 WW domain #1 (ARHGAP39 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103 KIAA1688 WW domain #2

    Plasmid

    #104278

    Purpose

    Bacterial expression of WW domain #2 from KIAA1688

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    KIAA1688 WW domain #2 (ARHGAP39 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103 IQGAP2 WW domain

    Plasmid

    #104280

    Purpose

    Bacterial expression of WW domain from IQGAP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    IQGAP2 WW domain (IQGAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103 ARHGAP27 WW domain #1

    Plasmid

    #104282

    Purpose

    Bacterial expression of WW domain #1 from ARHGAP27

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    ARHGAP27 WW domain #1 (ARHGAP27 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103 ARHGAP27 WW domain #2

    Plasmid

    #104283

    Purpose

    Bacterial expression of WW domain #2 from ARHGAP27

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    ARHGAP27 WW domain #2 (ARHGAP27 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHH0103 PDZK10 WW domain

    Plasmid

    #104285

    Purpose

    Bacterial expression of WW domain from PDZK10

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    PDZK10 WW domain (FRMPD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHH0103 ARHGAP9 WW domain

    Plasmid

    #104286

    Purpose

    Bacterial expression of WW domain from ARHGAP9

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    ARHGAP9 WW domain (ARHGAP9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHH0103 IQGAP3 WW domain

    Plasmid

    #104289

    Purpose

    Bacterial expression of WW domain from IQGAP3

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    IQGAP3 WW domain (IQGAP3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103 WWTR1 WW domain

    Plasmid

    #104261

    Purpose

    Bacterial expression of WW domain from WWTR1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWTR1 WW domain (WWTR1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103 FNBP4 WW domain #1

    Plasmid

    #104263

    Purpose

    Bacterial expression of WW domain #1 from FNBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP4 WW domain #1 (FNBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103 FNBP4 WW domain #2

    Plasmid

    #104264

    Purpose

    Bacterial expression of WW domain #2 from FNBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP4 WW domain #2 (FNBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHH0103 DRP2 WW domain

    Plasmid

    #104270

    Purpose

    Bacterial expression of WW domain from DRP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    DRP2 WW domain (DRP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hC73S-pEGFP-C1

    Plasmid

    #111540

    Purpose

    Expresses human KCNB1 C73S in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human C73S-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73S

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hC73D-pEGFP-N1

    Plasmid

    #111534

    Purpose

    Expresses human KCNB1 C73D in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human C73D-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73D

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. hC73A-DN-HA

    Plasmid

    #111531

    Purpose

    Expresses a conducting dominant neg in mammalian cells

    Depositor

    Federico Sesti

    Insert

    human C73A-W369C-Y384T-Kv2.1 + HA (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C73A-W369C-Y384T

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. hC73A-pEGFP-C1

    Plasmid

    #111539

    Purpose

    Expresses human KCNB1 C73A in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human C73A-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73A

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. hC73W-pEGFP-N1

    Plasmid

    #111537

    Purpose

    Expresses human KCNB1 C73W in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human C73W-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73W

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. hC73M-pEGFP-N1

    Plasmid

    #111535

    Purpose

    Expresses human KCNB1 C73M in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human C73M-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73M

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. His8:MBP-tev-Leu-eΔK-Flv

    Plasmid

    #83397

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid

    #83396

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid

    #83395

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pVP16-tev-PRT1-C195A

    Plasmid

    #83408

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    C195A substitution

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pVP16-tev-PRT1-C29A

    Plasmid

    #83407

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    C29A substitution

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pVP16-tev-PRT1-V28A

    Plasmid

    #83406

    Purpose

    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    V28A substitution

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid

    #87152

    Purpose

    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA1-gltA2-udhA-zwf guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge