We narrowed to 31,526 results for: Eng

1. pTK_BsmBI_LacZ_Citrine_Nanotag_17

   Plasmid

   #130518

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTK_BsmBI_LacZ_Citrine_Nanotag_11

   Plasmid

   #130517

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTH1-SaSrtA-KA

   Plasmid

   #64981

   Purpose

   Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108A

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MCV-hVam6p-

   Plasmid

   #32059

   Depositor

   Patrick Moore

   Article

   Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

   Insert

   MCV-hVam6p-
   

   Use

   Tags

   Expression

   Mutation

   TG to GC mutations at 3280-3281 (nt) of full cons…

   Promoter

   none from vector. Insert contains native promoter…

   Available since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTL-4

   Plasmid

   #52033

   Purpose

   Inducible expression of GST-unc43 in E. coli

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   Unc-43 (unc-43 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pTK-EdnrbE2-mCherry

   Plasmid

   #127772

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE2
   

   Use

   Enhancer reporter construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJKW1847

   Plasmid

   #174004

   Purpose

   Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

   Depositor

   Jing-Ke Weng

   Article

   Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

   Inserts

   PpCYP94D108
   ATR1 (ATR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTYB3-human PUM1-HD MUT1-1 (Q867E)-intein-CBD

   Plasmid

   #73301

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   Q867E

   Promoter

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. TU#1450_unc-53a_cDNA

   Plasmid

   #82361

   Purpose

   express unc-53a cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   unc-53 a isoform (unc-53 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFPQ3

    Plasmid

    #83558

    Purpose

    Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL2

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVC-Ds-DrU6a:sgRNA-Ds

    Plasmid

    #119069

    Purpose

    To clone sgRNA spacer sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFL-TK-AID3'UTR

    Plasmid

    #19986

    Depositor

    Nina Papavasiliou

    Article

    Teng et al Immunity. 2008 May . 28(5):621-9.

    Insert

    AID 3' UTR (Aicda Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pre_IV_CS6_4323_v1

    Plasmid

    #126859

    Purpose

    4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS6_4323_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L1-D544N

    Plasmid

    #15499

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-D544N (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Aspartic Acid 544 changed to Asparagine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. Tol2-lyzC-microRNA 146a-Dendra2

    Plasmid

    #97113

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    microRNA 146a Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tol2-lyzC-microRNA 199-3-Dendra2

    Plasmid

    #97119

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    microRNA 199 3 Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGGC001

    Plasmid

    #48824

    Purpose

    Provides WUS as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    WUS (WUS Mustard Weed)
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    internal BsaI recognition site removed by silent …

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L1-S542P

    Plasmid

    #15489

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-S542P (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Serine 542 changed to Proline

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pJT3

    Plasmid

    #98304

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TRPL3AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTYB3-human PUM1-HD MUT3-1 (C935N)-intein-CBD

    Plasmid

    #73304

    Purpose

    Expresses mutant human PUM1-HD

    Depositor

    Traci Hall

    Article

    Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    C935N

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

21. TU#1448_unc-73b_cDNA

    Plasmid

    #82362

    Purpose

    express unc-73b cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 b isoform (unc-73 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. human pirh2-(138-189)

    Plasmid

    #24881

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 138-189.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

23. L1-I219T

    Plasmid

    #15486

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-I219T (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoleucine 219 changed to Threonine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pVEA3-DR

    Plasmid

    #234800

    Purpose

    Mammalian expression of bovine arrestin-3 (D291R)

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    D291R

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3VEA3

    Plasmid

    #234796

    Purpose

    Mammalian expression of bovine arrestin-3

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVEA3-Tr

    Plasmid

    #234798

    Purpose

    Mammalian expression of truncated bovine arrestin-3 (1-393)

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    truncated (1-393)

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVEA3-RE

    Plasmid

    #234799

    Purpose

    Mammalian expression of bovine arrestin-3 (R170E)

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    R170E

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQAT44

    Plasmid

    #223416

    Purpose

    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQAT45

    Plasmid

    #223417

    Purpose

    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQAT47

    Plasmid

    #223419

    Purpose

    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYPQAT48

    Plasmid

    #223420

    Purpose

    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYPQAT49

    Plasmid

    #223421

    Purpose

    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQAT50

    Plasmid

    #223422

    Purpose

    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA was driven by separate ZmUbi1 promoter; Hygromycin for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQAT51

    Plasmid

    #223423

    Purpose

    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYPQAT52

    Plasmid

    #223424

    Purpose

    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYPQAT53

    Plasmid

    #223425

    Purpose

    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYPQAT54

    Plasmid

    #223426

    Purpose

    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-ecTadA8e-dLbCas12a-D156R-2x35s-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYPQAT33

    Plasmid

    #223405

    Purpose

    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQAT34

    Plasmid

    #223406

    Purpose

    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYPQAT35

    Plasmid

    #223407

    Purpose

    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pYPQAT36

    Plasmid

    #223408

    Purpose

    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYPQAT41

    Plasmid

    #223413

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYPQAT06

    Plasmid

    #223378

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLS-CMV-mP-luc

    Plasmid

    #213506

    Purpose

    Positive control vector for luciferase lentivirus-based enhancer assay

    Depositor

    Nadav Ahituv

    Article

    Deng et al Science. 2024 May 24;384(6698):eadh0559.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

    Plasmid

    #200877

    Purpose

    ZooTsy_TnsF expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy_TnsF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TnsF-Y584F

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSCT384: pSC101-Donor(ZooTsy)

    Plasmid

    #200875

    Purpose

    ZooTsy donor harboring KanR cargo.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSCT81: pTarget(ZooTsy)

    Plasmid

    #200876

    Purpose

    Target plasmid containing attachement site for ZooTsy.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy_comM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSCT315: pHelper(ZooTsy)_TnsF-Y584F

    Plasmid

    #200872

    Purpose

    Inducible expression of ZooTsy proteins.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TnsF-Y584F

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSCT375: pDonor(ZooTsy)_excision-reporter

    Plasmid

    #200873

    Purpose

    Reporter for ZooTsy excision.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSCT155: pHelper(ZooTsy)_ΔHTH

    Plasmid

    #200868

    Purpose

    Inducible expression of ZooTsy (except HTH) proteins.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    ZooTsy
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge