We narrowed to 7,692 results for: Lif;
-
Plasmid#184975PurposeExpress -Eco1 CAN1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
ExpressionYeastMutationCAN1 donor G444stopPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.111
Plasmid#184980PurposeTest effect of extending a1/a2 on TRP2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
ExpressionYeastMutationTRP2 donor E64stop, a1/a2 length extended to 27 bpPromoterGal7Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.037
Plasmid#184966PurposeExpress -Eco1 dead RT and ncRNA in yeasstDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
ExpressionYeastMutationHuman codon optimized RT, YXDD catalytic region m…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.112
Plasmid#184981PurposeExpress -Eco1 FAA1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
ExpressionYeastMutationFAA1 donor P233stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
ExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.002
Plasmid#184972PurposeExpress -Eco1 ADE2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
ExpressionYeastMutationADE2 donor P272stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.110
Plasmid#184979PurposeExpress -Eco1 TRP2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
ExpressionYeastMutationTRP2 donor E64stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
ExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_grh-1p-grh-1-gfp
Plasmid#177761PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
ExpressionWormAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_hsp-16.2p-grh-1-gfp
Plasmid#177762PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
ExpressionWormAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_Hyg_FLAG-BirA-eGFP-NLS2
Plasmid#170917PurposeExpresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull downDepositorInsertFLAG-BirA-eGFP-NLS2
TagsBirA-FLAGExpressionMammalianAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago2
Plasmid#170918PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2DepositorInsertBirA-FLAG
ExpressionMammalianPromoterlacAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HR-BirA-FLAG_Ago1
Plasmid#170919PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1DepositorInsertBirA-FLAG
ExpressionMammalianPromoterlacAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Pigb-V5
Plasmid#175160PurposeLentiviral expression of mouse Pigb-V5DepositorAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEGF-exon1
Plasmid#177261PurposeA knockout vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEGF-5’UTR
Plasmid#177260PurposeA knockout vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2Aneo-mRFP-FKBP-mSos1-linkercat
Plasmid#173858PurposeEncoding a part of the rapamycin-induced Ras activation system.DepositorInsertmRFP-FKBP-mSos1-linkercat
ExpressionMammalianAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAP12008
Plasmid#177036PurposeLevel 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCATDepositorInsertCauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dNrg1
Plasmid#173696PurposeA knockout vector for the dog Nrg1.DepositorInsertA gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEreg
Plasmid#173697PurposeA knockout vector for the dog Ereg.DepositorInsertA gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dTgfa
Plasmid#173698PurposeA knockout vector for the dog Tgfa.DepositorInsertA gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dHbegf
Plasmid#173699PurposeA knockout vector for the dog Hbegf.DepositorInsertA gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEgf
Plasmid#173700PurposeA knock-out vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-bleo-dEgf
Plasmid#173840PurposeA knock-out vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-puro-dHbegf
Plasmid#173841PurposeA knockout vector for the dog Hbegf.DepositorInsertA gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-neo-dTgfa
Plasmid#173842PurposeA knockout vector for the dog Tgfa.DepositorInsertA gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-hyg2-dEreg
Plasmid#173843PurposeA knockout vector for the dog Ereg.DepositorInsertA gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-puro-dEgfr
Plasmid#173844PurposeA knockout vector for the dog Egfr.DepositorInsertA gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisHbegf
Plasmid#173846PurposeEncoding dog Hbegf.DepositorInsertA cDNA of canis Hbegf.
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisTgfa
Plasmid#173847PurposeEncoding dog Tgfa.DepositorInsertA cDNA of canis Tgfa.
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-canisEreg
Plasmid#173848PurposeEncoding dog Ereg.DepositorInsertA cDNA of canis Tgfa.
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH–PB
Plasmid#169685PurposeAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody
Tagsmodified STIM1-PB tagExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E06: attB5r-pA-polyhedrinp>-attB1r
Plasmid#162948PurposeGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertAcMNPV polyhedrin promoter with upstream polyA sequence
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER-CAmut
Plasmid#155237Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
Tags6XHis-3XFlagExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176E
Plasmid#131203Purposemammalian expression, tet RDepositorAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_Y474F
Plasmid#131204Purposemammalian expression, tet RDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A
Plasmid#127786PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32 and Arg36 to AlaAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R438A-K441A
Plasmid#127791PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg4…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-TTM
Plasmid#127794PurposeExpresses the TTM/CYTH domain of ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 1-200)Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-ScSEIPIN-Ndelta
Plasmid#96985PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
Tags6xHis and GFPExpressionPlantMutationN-terminal 39 nucleotides were deleted from yeast…Promoter35SAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN2
Plasmid#96987PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
ExpressionYeastPromoterPhosphoglycerate Kinase (PGK) promoterAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3N-1C
Plasmid#96983PurposeExpress mutated Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
Tags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides from the N terminus…Promoter35SAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN1N-3C
Plasmid#96982PurposeExpress mutated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
Tags6xHis and GFPExpressionPlantMutationA segment of 108 nucleotides from the N terminus…Promoter35SAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mPar6a K19A
Plasmid#86149PurposeFLAG-mPar6a K19A - Does not bind to aPKCDepositorInsertPar6 (Pard6a Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationLysine to Alanine mutation K19APromoterLTRAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV HPV8 E6 del132-136
Plasmid#87658PurposeFlag/HA tagged HPV8 E6 del132-136DepositorInsertHPV 8E6
TagsFlag and HAExpressionMammalianMutationDeletion of aa132-136PromoterCMVAvailable SinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW301ParsR-Amp30T
Plasmid#78653PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyPromotersee insertAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo-NKX2-8
Plasmid#75090Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorInsertNKX2-8 (NKX2-8 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRevTre-mKLF5
Plasmid#41744DepositorInsertKlf5 (Klf5 Mouse)
ExpressionMammalianAvailable SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
refGFP1_pQE30
Plasmid#48644PurposeBact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFPDepositorInsertrefGFP1
Tags6xHisExpressionBacterialMutationrefGFP C147S, C204QAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only