We narrowed to 157,143 results for: Des

1. pDEST-CMV GFP-LC3B G120

   Plasmid

   #123116

   Purpose

   Expresses EGFP-LC3B G120 in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 121-125. Stop codon after G12…

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

   Plasmid

   #99261

   Purpose

   Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

   Depositor

   Alejandro Adam

   Article

   Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

   Insert

   Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
   

   Use

   Adenoviral

   Tags

   3x-Flag

   Mutation

   Y705F (contains a silent mutation: nucleotide 271…

   Promoter

   CMV

   Available Since

   Jan. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

   Plasmid

   #51252

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 789,805,817,848 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pDEST27-GST-ATXN1 FL(92Q)-S776D

   Plasmid

   #21757

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 92 Q(Glutamines) and…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDestTol2-actinb-kcnj10a-IRES-EGFP

   Plasmid

   #164947

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj10a (kcnj10a Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Promoter

   actinb (actb2) promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Desmoplakin I tension sensor (F40-based)

   Plasmid

   #118725

   Purpose

   The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

   Plasmid

   #56658

   Purpose

   expresses constitutively active human PKCi with a deletion on the PB1 domain

   Depositor

   Pedro Salas

   Article

   Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

   Insert

   Human PKCi (PRKCI Human)
   

   Use

   Lentiviral

   Tags

   V5/His

   Mutation

   A120E and deletion of PB1 domain (aa 24–98)

   Promoter

   CMV

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEJS507-pCDest2-noAcr-mTagBFP2-IRES

   Plasmid

   #85748

   Purpose

   Expresses mTagBFP2 only; used as a control

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-ATXN1 FL(30Q)-S776D

   Plasmid

   #21755

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pDestTol2-pax3a-kcnj13-IRES-EGFP

    Plasmid

    #164950

    Purpose

    Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Promoter

    pax3a promoter -5.4k

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestTol2-actinb-kcnj13-IRES-EGFP

    Plasmid

    #164941

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Promoter

    actinb (actb2) promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Desmoplakin II no force control (F40-based)

    Plasmid

    #118716

    Purpose

    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

    Plasmid

    #51254

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Asp

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-CMV GFP-GABARAPL1 G116A

    Plasmid

    #123118

    Purpose

    Expresses EGFP-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. hu-TLR4 299 snp-flag pDEST40

    Plasmid

    #42647

    Purpose

    destination vector for expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEF5/FRT-DEST EVC2dW-Flag

    Plasmid

    #41007

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1177-1220

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA 3.2/V5-DEST NET1A S46A

    Plasmid

    #69818

    Purpose

    Expresses NET1A-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S46A

    Promoter

    CMV

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

    Plasmid

    #113437

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF5/FRT/DEST-3xHA/mGli2 P5-6A

    Plasmid

    #51253

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only