We narrowed to 3,022 results for: ER

1. pEGFPC1-hVAP-A KD/MD

   Plasmid

   #104449

   Purpose

   Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K94 to D and M96 to D

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

   Plasmid

   #162382

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CAG

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

   Plasmid

   #236231

   Purpose

   AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET iGluSnFR3 v82

   Plasmid

   #175187

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v82
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 SIRT7_H187Y

   Plasmid

   #53151

   Purpose

   expression vector for catalytically inactive human Sirtuin7

   Depositor

   Katrin Chua

   Article

   Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

   Insert

   Sirt7 (SIRT7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H187Y, catalytic inactive

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-TFIIE

   Plasmid

   #171083

   Purpose

   Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   TF2E1 (GTF2E1 Human)
   TF2E2 (GTF2E2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

   Plasmid

   #236246

   Purpose

   Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   U6 and human Synapsin1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CMV-jGCaMP7c variant 1513

   Plasmid

   #105320

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106186

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106180

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV5

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

    Plasmid

    #162388

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2-elavl3-Voltron-ST

    Plasmid

    #119038

    Purpose

    Pan neuronal expression of soma-localized Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron-ST
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222497

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6p-1-UFBP1

    Plasmid

    #204558

    Purpose

    Expresses GST-tagged UFBP1 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFBP1 (DDRGK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

    Plasmid

    #236232

    Purpose

    AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mScarletI

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-NUP98a_IDR-EGFP

    Plasmid

    #232766

    Purpose

    Expresses dCas9-NUP98a_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98a_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-STAT-CA

    Plasmid

    #195576

    Purpose

    Expresses STAT3 constitutively active mutant

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    changed Alanine 662 to Cysteine and Asparagine 66…

    Promoter

    CMV enhancer and promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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