We narrowed to 9,252 results for: SUB;

1. pACT2-AP-3 σ3A

   Plasmid

   #198347

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-3 σ3A
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACT2-AP-3 σ3B

   Plasmid

   #198348

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-3 σ3B
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVRC-N-Venus-V150A-CC-Di

   Plasmid

   #190707

   Purpose

   Encodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.

   Depositor

   Nigel Savery

   Article

   Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

   Insert

   N-Venus-V150A-CC-Di
   

   Use

   Tags

   Expression

   Mutation

   V150A

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVRC-N-Venus-V150A

   Plasmid

   #190708

   Purpose

   Control plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.

   Depositor

   Nigel Savery

   Article

   Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

   Insert

   N-Venus-V150A
   

   Use

   Tags

   Expression

   Mutation

   V150A

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCAS myr-c-P3k

   Plasmid

   #196373

   Purpose

   Oncogenic with addition of a myristylation signal and expresses in avian cells

   Depositor

   Peter Vogt

   Article

   Chang et al Science. 1997 Jun 20;276(5320):1848-50.

   Insert

   Myr-c-P3k-FLAG
   

   Use

   Retroviral; Avian

   Tags

   flag and myristoylation

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7PI-Sce ESARC D326A

   Plasmid

   #195609

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T3593C, A3601C, T3602G

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-SaCas9-U6-sgKcna2

   Plasmid

   #192796

   Purpose

   Encodes SaCas9 and an sgRNA targeting mouse Kcna2

   Depositor

   Marta Soden

   Article

   Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

   Insert

   sgKcna2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgKcna6

   Plasmid

   #192797

   Purpose

   Encodes SaCas9 and an sgRNA targeting mouse Kcna6

   Depositor

   Marta Soden

   Article

   Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

   Insert

   sgKcna6
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_rSERT_X5C_S277C

   Plasmid

   #190177

   Purpose

   For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

   Depositor

   Gary Rudnick

   Article

   Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

   Insert

   rat serotonin transporter (Slc6a4 Rat)
   

   Use

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ND6-L1397-N_Q1310A_right

    Plasmid

    #187849

    Purpose

    expresses the right-side half ND6-Q1310A in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ND6-L1397-N_Q1310A_left

    Plasmid

    #187848

    Purpose

    expresses the left-side half ND6-Q1310A in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N(Q1310A)–UGI–SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

    Plasmid

    #192113

    Purpose

    Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Insert

    SARS-CoV-2-NTD-BA.4/5 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KEE

    Plasmid

    #187419

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    PH and p10

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. hp-66%GC-40bp

    Plasmid

    #169200

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    66%GC-40bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hp-75%GC-20bp

    Plasmid

    #169201

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    75%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hp-75%GC-40bp

    Plasmid

    #169202

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    75%GC-40bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hp-100%GC-20bp

    Plasmid

    #169203

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    100%GC-20bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hp-50%GC-Left-half-120bp

    Plasmid

    #169204

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-Left-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hp-50%GC-Right-half-120bp

    Plasmid

    #169205

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50%GC-Right-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hp-50-0-50%GC-Left-half-120bp

    Plasmid

    #169206

    Purpose

    Force-activating hairpin for 3'-to-5' helicase

    Depositor

    Tom Perkins

    Article

    Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

    Insert

    50-0-50%GC-Left-half-120bp-hairpin
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge