We narrowed to 14,200 results for: cas9

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pX330.puro

Plasmid

#110403

Purpose

CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

Depositor

Sandra Martha Gomes Dias

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pMS2M-PH-gagpol-D64V

Plasmid

#166031

Purpose

Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

Depositor

Yujia Cai

Publication

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

MS2-Gag-Pol

Use

Lentiviral

Mutation

D64V mutation within the integrase within Pol

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAT15546_nABE8e-SuperFi-Cas9

Plasmid

#184376

Purpose

Mammalian expression of SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax

Plasmid

#222138

Purpose

Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 (cas9 Synthetic)

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Publication

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO and U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Publication

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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UAS-u(XL)Cas9

Plasmid

#127386

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Publication

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XL)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Promoter

EFS

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLQ-KO-tgt50

Plasmid

#126578

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Publication

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50

Use

CRISPR

Expression

Bacterial

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gLacZ.Cas9-T2A-GFP

Plasmid

#170361

Purpose

Negative control plasmid used for Crispr/cas9 based disruption.

Depositor

Johan Jakobsson

Publication

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Promoter

U6

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-VQR-P2A-Puro

Plasmid

#110848

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-P2A-Puro

Plasmid

#110852

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRERRA-PGK-Puro

Plasmid

#110859

Purpose

Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRERRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, G1218R, R1335E, T1337R, and NLS sequence …

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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