161,439 results

1. pLVX-TetOne-Puro-mitoEcSTH-FLAG

   Plasmid

   #218629

   Purpose

   This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoEcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOCC305

   Plasmid

   #118898

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and TwinStrep-MB…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRevTRE MKL1

   Plasmid

   #19846

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

4. AICSDP-52: HIST1H2BJ-mEGFP

   Plasmid

   #109121

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ

   Depositor

   Allen Institute for Cell Science

   Insert

   HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pPSdown

   Plasmid

   #149417

   Purpose

   Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PresentER-SIINFEKL (GFP)

   Plasmid

   #102944

   Purpose

   PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   SIINFEKL (OVAL Chicken, Synthetic)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   OVA257-264

   Promoter

   LTR

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mNeonGreen-KDEL

   Plasmid

   #226568

   Purpose

   Encodes mNeonGreen with KDEL ER lumenal target sequence

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   KDEL (ER lumenal target sequence)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPSup2

   Plasmid

   #149418

   Purpose

   Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Flag-hMcl-1

   Plasmid

   #25392

   Depositor

   Roger Davis

   Insert

   MCL1 (MCL1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMK347 (BSD-P2A-mAID)

    Plasmid

    #121181

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge