We narrowed to 5,630 results for: donor
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Plasmid#207537PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cellsDepositorInsertHaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_His2-GFP11
Plasmid#153511PurposeHis2-GFP11 C-terminal Tag Donor PlasmidDepositorInsertHis2-GFP11
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-lox2272
Plasmid#154828PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietinDepositorInsertcoEPO
UseCre/LoxExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATM-Halo HRD
Plasmid#207082PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
TagsHaloTagExpressionMammalianPromoterNoneAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mCherry
Plasmid#219657PurposePermits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPP5741
Plasmid#128722PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcV-hopV1
ExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-078:G3BP1-mEGFP
Plasmid#193920PurposeHomology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1DepositorInsertG3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEED-OLLAS:HA
Plasmid#219647PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest methodDepositorInsertOLLAS:HA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT2_14_GlyOx
Plasmid#177711PurposePlasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-PAK-mMaroon
Plasmid#84361PurposeFRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-PAK-mMaroon
TagsmMaroon flanking PAK domain on N and C terminusExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOC1 Halo-Actb KI
Plasmid#183426PurposeCreON knock-in for Halo-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and Halo donor
UseCRISPRAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRE57-Ter
Plasmid#170635PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, Ter cassette
ExpressionBacterialAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
ExpressionMammalianPromoterNoneAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-RTK-mMaroon
Plasmid#84359PurposeFRET acceptor for RhoA donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-Rtk--mMaroon
TagsmMaroon flanking Rtk domain on N and C terminusExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2 HRD
Plasmid#207544PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cellsDepositorInsertHaloTag flanked by human WIPI2 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYZ172
Plasmid#98409PurposeDonor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsupstream homologous region to delete lys9 in genome by recombination
downstream homologous region to delete lys9 in genome by recombination
UseCRISPR and Synthetic BiologyAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-UGCG-tdTomato
Plasmid#225879PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertionDepositorInserttdTomato
UseCRISPRExpressionMammalianAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only