We narrowed to 2,414 results for: organization Addgene

1. pcDNA 3.1 AURKA deltaNter

   Plasmid

   #157749

   Purpose

   Expression of AURKA without Nter in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   the first 30AA of Aurora A are lacking

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-human HoxB4-IRES-YFP

   Plasmid

   #91889

   Purpose

   Retroviral expression of human HoxB4

   Depositor

   Brian Sorrentino, Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   HoxB4 (HOXB4 Human)
   

   Use

   Retroviral

   Tags

   IRES-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV gag

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRPML1-mCherry

   Plasmid

   #135189

   Purpose

   Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TRPML1 (Mcoln1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-FNLS-P2A-GFP-PGK-Puro

   Plasmid

   #110869

   Purpose

   Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-VQR-P2A-GFP-PGK-Puro

   Plasmid

   #110861

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV 4xSTAR-mTagBFP2-blast

   Plasmid

   #125239

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTagBFP2 (codon optimized)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/avitag-apex2-v5-fkbp8

   Plasmid

   #154460

   Purpose

   expresses avitag-apex2-v5-fkbp8/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   avitag-apex2-v5-fkbp8 (FKBP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F, T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. W45A mutant of H3K9me3 biosensor

   Plasmid

   #120808

   Purpose

   FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 AURKA Lys162Met

    Plasmid

    #157751

    Purpose

    Expression of kinase-dead AURKA K62M in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-FAB(PX-LD)

    Plasmid

    #214422

    Purpose

    Detection of peroxisome-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and FKBP-V5-RspAN

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3/flag-pbira(1-g78)-sec61b

    Plasmid

    #154455

    Purpose

    expresses flag-pbira(1-g78)-sec61b/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pbira(1-g78)-sec61b (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-HF1RA-P2A-GFP-PGK-Puro

    Plasmid

    #110866

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204189

    Purpose

    Doxycycline inducible lentiviral vector for human GATA6 overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-xBE4Gam-P2A-Puro

    Plasmid

    #112676

    Purpose

    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE4Gam
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-xBE3-P2A-Puro

    Plasmid

    #110870

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2K-CAGGS-mouse Gfi1-IRES-GFP

    Plasmid

    #91893

    Purpose

    Mammalian expression of mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pPB CAG Cdk6 R31C-IRES-EGFP

    Plasmid

    #153946

    Purpose

    piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Cdk6 (Cdk6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R31C

    Promoter

    CAG

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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