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pX459V2.0-HypaCas9

Plasmid

#108294

Purpose

pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGE010

Plasmid

#71536

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pH-EF1α-DualPE

Plasmid

#236417

Purpose

For plant prime editing including large DNA fragment editing in Solanum lycopersicum or other dicotyledons

Depositor

Zong Yuan

Article

Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:

Insert

EF1α promoter

Use

CRISPR

Expression

Plant

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Calmodulin1 (CaM)

Plasmid

#240202

Purpose

Calmodulin-1 for CaMK2-alpha sensor assays in mammalian cell lines

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

calmodulin-1 (Calm1 Mouse)

Tags

None

Expression

Mammalian

Promoter

pCAG

Available Since

July 31, 2025

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

Plasmid

#102886

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-IGK-dAPEX2-KDEL

Plasmid

#117183

Purpose

Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

IGK-dAPEX2-KDEL

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMIA22

Plasmid

#214448

Purpose

Expresses BxbI-BP-NLS

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

BxbI

Tags

BP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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