We narrowed to 10,315 results for: 158

1. pAN1646

   Plasmid

   #127205

   Purpose

   pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. p27-mVenus reporter (PB)

   Plasmid

   #190731

   Purpose

   The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   mVenus
   p27
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   p27-F62A-F64A

   Promoter

   EF1α

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2-P2A-GFP

   Plasmid

   #132776

   Purpose

   Prime editing in mammalian cells with co-translational GFP expression

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2-P2A-GFP
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

   Plasmid

   #161580

   Purpose

   Express Kir2.1 in cells not expressing Cre.

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

   Plasmid

   #135955

   Purpose

   Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Prime editor v2 (PE2) N-terminal
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-ChRmine-mScarlet

   Plasmid

   #137158

   Purpose

   Viral expression of ChRmine-p2a-mScarlet

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   ChRmine-p2a-mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIDK-HDAC1fl

   Plasmid

   #109158

   Purpose

   Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. EXT1- D164A-pLJC2-3XFLAG

   Plasmid

   #215828

   Purpose

   Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid

    #160458

    Purpose

    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Inserts

    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    

    Use

    Insertion of a fluorescent reporter through homol…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. DRH323: pAAV_hSyn-QuasAr2

    Plasmid

    #107705

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. YA1532: pAAV_ CAG-FLEX-paQuasAr3

    Plasmid

    #107702

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ID1-pLVXT

    Plasmid

    #119280

    Purpose

    Doxycycline-inducible mouse ID1 from 806 cells

    Depositor

    Joan Massague

    Article

    Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer20 ALPK1 WT

    Plasmid

    #231585

    Purpose

    ALPK1 WT gene under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-SwitchON-sgVRK1

    Plasmid

    #199638

    Purpose

    Tamoxifen-inducible expression of sgRNA targeting VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YA1531: pAAV_CAG-FLEX-QuasAr3

    Plasmid

    #107701

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

    Plasmid

    #166804

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S1213A

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. YA1629: pAAV_CAG-FLEX-paQuasAr3-s

    Plasmid

    #107703

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAPM-D4 miR30-AGO2 ts1

    Plasmid

    #115853

    Purpose

    AGO2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO2 shRNA (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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