We narrowed to 13,743 results for: TIM

1. pMiniT:SEP-GluN1 TKIT donor

   Plasmid

   #169427

   Purpose

   Donor DNA for TKIT knockin of SEP to NR1 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato-Lifeact-7

   Plasmid

   #54528

   Purpose

   Localization: Actin, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Type

   Empty backbone

   Use

   Tags

   tdTomato-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_AncBE4max_3xHA

   Plasmid

   #112097

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_3xHA
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_NES_HaloTag9

   Plasmid

   #175537

   Purpose

   Strict cytosolic expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   NES-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CeNL/pcDNA3

   Plasmid

   #85199

   Purpose

   Cyan color variant of bright luminescent protein

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   Cyan enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CeNL_pRSETb

   Plasmid

   #89510

   Purpose

   Cyan color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. DL033:cFos::eGFP-iPAK4

   Plasmid

   #177883

   Purpose

   eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   cFos

   Available since

   July 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCE1

   Plasmid

   #174434

   Purpose

   Candida optimized monomeric eGFP with RIPLING linker sequence.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   Candida optimized monomeric GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWRS3001_VCR1_WCR3

    Plasmid

    #192206

    Purpose

    VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Insert

    VCR1-WCR3 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOS017: ratiometric pHluorin (cytosolic)

    Plasmid

    #163052

    Purpose

    ratiometric pHluorin (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFN18A-HaloTag-SpyCatcher

    Plasmid

    #206041

    Purpose

    HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    HaloTag and SpyCatcher-8His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNB769

    Plasmid

    #60138

    Purpose

    Red click beetle luciferase from Promega driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. [pSK600] pRK5 HA-Nprl2(R78A)

    Plasmid

    #136146

    Purpose

    mammalian expression of Nprl2

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al J Biol Chem. 2019 Feb 22;294(8):2970-297

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R78A

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-oChIEF-tdTomato

    Plasmid

    #50977

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. ReNL_pRSETb

    Plasmid

    #89536

    Purpose

    Red color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pASTA3

    Plasmid

    #24657

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eMagAF-EGFP-Mito

    Plasmid

    #162243

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-OMP25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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