165,775 results

1. pHAGE-TO-dCas9-GFP

   Plasmid

   #64106

   Purpose

   Expression of Sp dCas9-GFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   sfGFP

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GRM1-DuET

   Plasmid

   #213300

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM1 (GRM1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LARS_pNIC-Bio3

   Plasmid

   #153052

   Purpose

   Expression construct for producing Avi tagged Leucyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   LARS (LARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Not full length protein

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1/ZMYM2-FGFR1

   Plasmid

   #167486

   Purpose

   Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

   Depositor

   Nicholas Cross

   Article

   Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

   Insert

   ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
   

   Expression

   Mammalian

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-TRE-DIO-tdTomato

   Plasmid

   #99116

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-tdTomato
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-Parkin-IRES-zeo

   Plasmid

   #61727

   Purpose

   Expression of human Parkin fused to mCherry

   Depositor

   Douglas Green, Stephen Tait

   Article

   Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

   Insert

   Parkin (PRKN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SF3B3-1

   Plasmid

   #156004

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SF3B3 (SF3B3 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

   Plasmid

   #196415

   Purpose

   Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smMyc
   

   Use

   AAV; Astrocyte-selective

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFB1_hMSH6

    Plasmid

    #129424

    Purpose

    Expression human MSH6 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6 (MSH6 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge