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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 30,511 results for: ica

Showing: 3091 - 3120 of 30511 results

  1. pCast

    Plasmid
    #200169
    Purpose
    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme
    Depositor
    Yong-Su Jin
    Article
    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200169-plasmid-map-sequence-id-396442

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  2. pKD233.7-3

    Plasmid
    #90955
    Purpose
    Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bba_J23114, PltetO-1, and PttrB185-269
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    90955-plasmid-map-sequence-id-178326

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  6. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

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  10. pFEndoNA

    Plasmid
    #174724
    Purpose
    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174724-plasmid-map-sequence-id-343066

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  13. pL0-MtPT4Pro

    Plasmid
    #159414
    Purpose
    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtPT4 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159414-plasmid-map-sequence-id-323169

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  14. pL0-MtBCP1Pro

    Plasmid
    #159415
    Purpose
    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtBCP1 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159415-plasmid-map-sequence-id-323165

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  16. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

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  17. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  18. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  19. SP6-VEE-IRES-Puro

    Plasmid
    #58971
    Purpose
    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Type
    Empty backbone
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58971-plasmid-map-sequence-id-91402

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  27. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

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  29. pL0_AC-CEN_ARS_HIS

    Plasmid
    #202129
    Purpose
    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    CEN_ARS_HIS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ARS sequence mutated to remove sapI site for dome…
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202129-plasmid-map-sequence-id-405565

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Showing: 3091 - 3120 of 30511 results