We narrowed to 2,359 results for: Efs

1. pEF-pIgH-ESCargo*

   Plasmid

   #140159

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-DHB-mTurq2

   Plasmid

   #212667

   Purpose

   Express tagged DHB

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   DHB (HELB Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-pIgH-ESCargo(FTV,Orange)

   Plasmid

   #140161

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHC01

   Plasmid

   #106900

   Purpose

   Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

   Depositor

   Jonathan Silberg

   Article

   Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

   Inserts

   LasR
   Methyl Halide Transferase
   Ethylene Forming Enzyme
   Red Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

   Plasmid

   #166411

   Purpose

   express hPOT1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   ef-1a

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL0_EF-PtBle

   Plasmid

   #202180

   Purpose

   Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   ShBle expression cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. BirA*-HA-NES

   Plasmid

   #214473

   Purpose

   Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   BirA(R118G)
   

   Use

   Lentiviral; Gateway

   Tags

   HA tag and NES (Nuclear Exclusion Sequence)

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   EF-1a

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-NES-fRGECO1

   Plasmid

   #125247

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Use

   Tags

   NES signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL0_EF-H4

   Plasmid

   #202178

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pL0_EF-fcpA

    Plasmid

    #202164

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpA terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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