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pMH185

Plasmid

#122839

Purpose

VP64 activator domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Publication

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

VP64 transcriptional activator domain

Use

Golden gate compatible cloning vector

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL2148

Plasmid

#71208

Purpose

pT181 Sense Expression Plasmid

Depositor

Julius Lucks

Publication

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pT181 Sense

Use

Synthetic Biology

Promoter

BBa_J23119

Available since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Publication

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Promoter

T7

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1

Plasmid

#60385

Purpose

Expression of yeast alpha tubulin (TUB1) using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-EGFPFL

Plasmid

#46310

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFPFL DNA-1
linker
EGFPFL DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-EGFP100

Plasmid

#46309

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFP100 DNA-1
linker
EGFP100 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSMP-SMYD2_3

Plasmid

#36351

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD1_1

Plasmid

#36365

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD1_2

Plasmid

#36366

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pcDNAintron-EBOV-GP

Plasmid

#215692

Purpose

Produces the Ebola virus glycoprotein

Depositor

Melinda Brindley

Publication

Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBOV-GP

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EC67844

Plasmid

#209514

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF14_IRES-mCherry

Plasmid

#198384

Purpose

RNF14 expression

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF14 (RNF14 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF14 C417A_IRES-mCherry

Plasmid

#198385

Purpose

RNF14 C417A expression

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF14 (RNF14 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

catalytic cysteine mutation (C417A)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-TWIST2 neo

Plasmid

#197936

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Publication

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F271-OKT3

Plasmid

#190682

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

Depositor

Scott McComb

Publication

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLG327

Plasmid

#128797

Purpose

Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

Depositor

Christopher Voigt

Publication

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl(s)

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-p65-HSF1-Blast

Plasmid

#192652

Purpose

3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-p65-HSF1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV Aquamarine

Plasmid

#157777

Purpose

Expresses cyan fluorescent Aquamarine in cells

Depositor

Marc Tramier

Publication

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-i53

Plasmid

#119002

Purpose

Mammalian expression of i53 (53BP1) inhibitor and BFP

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

i53

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-TKBsd-L2

Plasmid

#141009

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKBsd

Expression

Mammalian

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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