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  2. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid
    #83107
    Purpose
    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
    Depositor
    Andrew Brooks
    Inserts
    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    Use
    Lentiviral
    Tags
    2A self cleaving peptide
    Expression
    Mammalian
    Mutation
    R24C and p64 isoform
    Promoter
    hPGK and hPGK (single transcript)
    Available since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83107-plasmid-map-sequence-id-330062

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  4. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109428-plasmid-map-sequence-id-212894

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  7. pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

    Plasmid
    #160619
    Purpose
    Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    P35S
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160619-plasmid-map-sequence-id-322017

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  9. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid
    #215014
    Purpose
    Express an autophagy (LC3) reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    MAP1LC3B (Map1lc3b Rat)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215014-plasmid-map-sequence-id-429267

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Showing: 2101 - 2120 of 28370 results