We narrowed to 2,463 results for: T2A

1. pYPQ269A2

   Plasmid

   #164714

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
   

   Use

   CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ269D

   Plasmid

   #164717

   Purpose

   Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
   

   Use

   CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

   Plasmid

   #172195

   Purpose

   AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   transcription regulatory domain removed

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRT2-H2B-EGFP-V5-2A (IG157)

   Plasmid

   #99623

   Purpose

   To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   H2B-EGFP-V5
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRT1-Mb2YFP-2A (IG153)

   Plasmid

   #99619

   Purpose

   To clone gene of interest downstream of FRT1-Mb2YFP-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2EYFP
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRT2-Mb2Tomato-2A (IG154)

   Plasmid

   #99620

   Purpose

   To clone gene of interest downstream of FRT2-Mb2Tomato-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2Tomato
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRT3-HA-H2B-Cerulean-2A (IG158))

   Plasmid

   #99624

   Purpose

   To clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   HA-H2B-Cerulean
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD

   Plasmid

   #226282

   Purpose

   Tetracycline-inducible expression of Isl1 and Lhx3 for direct reprogramming of canine fibroblasts to induced-motor neurons

   Depositor

   Susannah Sample

   Article

   Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

   Insert

   ISL1:T2A:LHX3 (ISL1, LHX3 Human)
   

   Use

   Lentiviral

   Tags

   Isl1 and Lhx3 linked via T2A

   Expression

   Mammalian

   Available Since

   Nov. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-U6-CNCB_sgEIF3D-1

   Plasmid

   #236765

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #1 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   EIF3D sgRNA-1 (EIF3D Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter and mouse U6 promoter, CAG prom…

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid

    #233046

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid

    #233045

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-hL1-5'_3.3kb plasmid

    Plasmid

    #226006

    Purpose

    Plasmid used to generate DNA-FISH probe targeting at human L1HS

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    Human L1 element 3.3kb sequences at the 5' end
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

    Plasmid

    #194226

    Purpose

    Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 1 HITI-2c ACTB

    Plasmid

    #206269

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HITI-2c donor
    

    Use

    CRISPR; Multimate/gateway entr 1

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166133

    Purpose

    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166134

    Purpose

    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

    Plasmid

    #172208

    Purpose

    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

    Plasmid

    #172200

    Purpose

    AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    transcription regulatory domain removed

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

    Plasmid

    #172205

    Purpose

    AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge