We narrowed to 165,567 results for: addgene

1. pN-PURO-PTP

   Plasmid

   #51711

   Purpose

   contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

   Depositor

   Arthur Gunzl

   Article

   Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

   Type

   Empty backbone

   Use

   T. brucei genome integration vectors

   Tags

   PTP (ProtC-TEV-ProtA)

   Available Since

   March 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. M4758 KanMX::URA3 Disruptor Converter

   Plasmid

   #51688

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to URA3

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   URA3 (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Expression

   Yeast

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR_eGFP_314

   Plasmid

   #111822

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 294-314
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. B1979

   Plasmid

   #59181

   Purpose

   Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

   Depositor

   Craig Mello

   Article

   Kim et al Genetics. 2014 May 30. pii: genetics.114

   Insert

   Blasticidin S resistance
   

   Expression

   Worm

   Promoter

   rpl-28

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3689

   Plasmid

   #154111

   Purpose

   miniMos single component RMCE landing site vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
   

   Use

   Minimos

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MXS_CMV::NeoR-bGHpA

   Plasmid

   #62437

   Purpose

   MXS_chaining vector with CMV::NeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Neomycin with CMV Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CXXC1

   Plasmid

   #32045

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CXXC1 (CXXC1 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   CXXC domain (amino acids residues 161-222)

   Available Since

   Jan. 3, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. MXS_PGK::PuroR-bGHpA

   Plasmid

   #62440

   Purpose

   MXS_chaining vector with PGK::PuroR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Puromycin with PGK Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2369

    Plasmid

    #36780

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Right (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2220

    Plasmid

    #36679

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    RECQL4-TALEN_Left (Recql4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. M4753 KanMX::ADE2 Disruptor Converter

    Plasmid

    #51683

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to ADE2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    ADE2 (ADE2 Budding Yeast)
    

    Use

    Yeast marker swap

    Expression

    Yeast

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. MXS_MCS1

    Plasmid

    #62395

    Purpose

    MXS_chaining vector with MCS1

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDpsTE-Ypet

    Plasmid

    #98277

    Purpose

    mammalian expression of DpsTE-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Dps
    

    Tags

    Ypet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBIP1::eYFP

    Plasmid

    #135231

    Purpose

    UPR signaling reporter (upregulates eYFP expression under UPR conditions)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    pBiP1
    

    Tags

    eYFP

    Expression

    Plant

    Promoter

    BiP1

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MXS_CMV::tTA2-bGHpA

    Plasmid

    #62447

    Purpose

    MXS_chaining vector with CMV::tTA2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tetracycline transactivator with CMV promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TAL2456

    Plasmid

    #36845

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    VHL-TALEN_Left (Vhl Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-U6-CB2gRNA-CBh-mCherry

    Plasmid

    #91948

    Purpose

    Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP64T-dnWnt-11

    Plasmid

    #16857

    Depositor

    Jim Smith

    Article

    Tada et al Development. 2000 May . 127(10):2227-38.

    Insert

    dnWnt-11 (wnt11b.L Frog)
    

    Use

    Xenopus expression

    Mutation

    amino acids 1-282. Dominant Negative.

    Available Since

    May 27, 2008

    Availability

    Academic Institutions and Nonprofits only