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pcDNA3.1hisA cdc25c S216A

Plasmid

#10968

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c S216A (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Mutation

S216A. Serine to Alanine substitution at aa216.

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only
pMyo-2::oxCerulean-oxVenus::Lgg-1

Plasmid

#68043

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-2::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUCSh5-2300

Plasmid

#171788

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pNVLTv2

Plasmid

#169245

Purpose

Generic suicide vector for knockouts and integrations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_19

Plasmid

#64045

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gRG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pVP64

Plasmid

#22287

Depositor

Louise Prakash

Article

Pages et al Genes Dev. 2009 Jun 15. 23(12):1438-49.

Insert

REV1 (REV1 Budding Yeast)

Tags

4xHA

Expression

Yeast

Available Since

April 22, 2010

Availability

Academic Institutions and Nonprofits only
pRS303 prp8-101

Plasmid

#111307

Purpose

prp8-101

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-101 (E1960K)

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pOX + kqt3

Plasmid

#16138

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-3 Nematode)

Use

Xenopus oocyte expression

Mutation

lacks aa1-16 compared to GenBank reference AY5729…

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pAGM62624

Plasmid

#169094

Purpose

Low copy MoClo level 2 binary vector for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRB37

Plasmid

#72869

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pVG48

Plasmid

#164706

Purpose

rtTA under the control of PGK1 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

PGK1pr - rtTA - CYC1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGKO1

Plasmid

#63611

Purpose

a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Binary fungal vector

Promoter

Lac Promoter

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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Mouse ZP3 (JD#47)

Plasmid

#14646

Depositor

Jurrien Dean

Article

Ringuette et al Dev Biol. 1988 Jun . 127(2):287-95.

Insert

ZP3 (Zp3 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
pRS405 VAC8-RFP

Plasmid

#166841

Purpose

Expresses Vac8-RFP in yeasts

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

RFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAF1-VP64

Plasmid

#51427

Purpose

Artificial transcription factor with VP64

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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p169 pCMV/betaAc-EGFP/beta-gal

Plasmid

#8388

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP beta-gal

Use

Cre/Lox; Beta-gal

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pVW284

Plasmid

#139494

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW300

Plasmid

#139495

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pTEF PP7∆FG-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pTEF

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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ZE3 Wnt-8

Plasmid

#17044

Depositor

Randall Moon

Article

Kelly et al Development. 1995 Jun. 121(6):1787-99.

Insert

wnt-8 full length (wnt8a Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only