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Showing: 4241 - 4280 of 8321 results

  2. pcDNA3.1_hSMAD4

    Plasmid
    #212710
    Purpose
    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens SMAD4 (SMAD4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212710-plasmid-map-sequence-id-448252

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  3. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid
    #172479
    Purpose
    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172479-plasmid-map-sequence-id-338913

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  4. pMT-IMD-V5-His

    Plasmid
    #63748
    Purpose
    Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    immune deficiency (imd Fly)
    Use
    Tags
    V5-6XHis
    Expression
    Insect
    Mutation
    *see comment below
    Promoter
    pMT
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63748-plasmid-map-sequence-id-108846

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  5. pAG426GAL-SARS-CoV-2_PLP

    Plasmid
    #203509
    Purpose
    Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker
    Depositor
    Alejandro Chavez
    Insert
    SARS-CoV-2 PLP (ORF1ab )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    203509-plasmid-map-sequence-id-402753

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  6. hMYOG-3xHA-IRES-hrGFP (#324)

    Plasmid
    #78341
    Purpose
    Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOG (MYOG Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid
    #185060
    Purpose
    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185060-plasmid-map-sequence-id-375730

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  8. PTPN7

    Plasmid
    #38945
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN7 (PTPN7 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. SIRT6

    Plasmid
    #41565
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-353
    Promoter
    T7
    Available since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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  11. pFR022

    Plasmid
    #134228
    Purpose
    bacterial expression of KNL1
    Depositor
    Iain Cheeseman
    Insert
    hKNL1 aa 2106-2316 (KNL1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    134228-plasmid-map-sequence-id-271855

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  13. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid
    #185699
    Purpose
    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter
    Depositor
    Lynette Lim
    Inserts
    Tdtomato,
    Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    mDlx
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185699-plasmid-map-sequence-id-366333

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  14. pT7-7 asyn S87E/S129E

    Plasmid
    #36064
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S87E/S129E (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S87E/S129E
    Promoter
    T7
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36064-plasmid-map-sequence-id-44521

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  15. PRDM11

    Plasmid
    #32858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM11 (PRDM11 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 79-314
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. TMPRSS2 g1 + Cas9

    Plasmid
    #153015
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153015-plasmid-map-sequence-id-298676

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  17. pAAV-synP-FLEX-TVA66T-EGFP-B19G

    Plasmid
    #64097
    Purpose
    Expresses TVA66T-EGFP-B19G under the synapsin promotor
    Depositor
    Ian Wickersham
    Insert
    TVA66T-EGFP-B19G
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    synapsin
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    64097-plasmid-map-sequence-id-107238

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  18. pDONR221-CTSL

    Plasmid
    #158444
    Purpose
    Gateway Cloning compatible entry vector for the human CTSL gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSL (CTSL Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158444-plasmid-map-sequence-id-308250

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  19. pcDNA3-FLAG-DDB1 (1140H)

    Plasmid
    #174849
    Purpose
    Expression of DDB1
    Depositor
    Helen Morrison
    Insert
    DDB1 (DDB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    174849-plasmid-map-sequence-id-344539

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  20. pSBtet-Bla/PAG1-GFP

    Plasmid
    #198220
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198220-plasmid-map-sequence-id-389402

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  21. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dTET1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104413-plasmid-map-sequence-id-204141

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  22. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid
    #78332
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. mEos4b-CenpA-C-22

    Plasmid
    #62775
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Loren Looger, Michael Davidson
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62775-plasmid-map-sequence-id-173170

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  24. TLCV2 sgAAVS1

    Plasmid
    #127099
    Purpose
    Encodes gRNA for human AAVS1.
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127099-plasmid-map-sequence-id-246497

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  25. PHGDH

    Plasmid
    #38841
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHGDH (PHGDH Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. pGL3-IL-4

    Plasmid
    #40570
    Depositor
    Mark Kaplan, Alexander Dent
    Insert
    IL-4 promoter (Il4 Mouse)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    contains 250 bp of the proximal mouse IL-4 promot…
    Promoter
    mouse IL-4
    Available since
    Oct. 12, 2012
    Availability
    Academic Institutions and Nonprofits only

  27. MAGEA4

    Plasmid
    #39001
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA4 (MAGEA4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39001-plasmid-map-sequence-id-218943

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  28. pLEX307-TMPRSS4-G418

    Plasmid
    #158474
    Purpose
    Constitutive lentiviral expression of human TMPRSS4.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS4 (TMPRSS4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158474-plasmid-map-sequence-id-308141

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  29. pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)

    Plasmid
    #44071
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44071-plasmid-map-sequence-id-61667

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  30. hMYF5-3xHA-IRES-hrGFP (#397)

    Plasmid
    #78331
    Purpose
    Overexpression of human MYF5 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYF5 (MYF5 Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid
    #44089
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44089-plasmid-map-sequence-id-61674

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  32. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. SETD3

    Plasmid
    #32873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD3 (SETD3 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pKM343

    Plasmid
    #69762
    Purpose
    Retroviral expression of CENP-I
    Depositor
    Iain Cheeseman
    Insert
    CENP-I (CENPI Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  36. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. RPS6KA1

    Plasmid
    #39021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RPS6KA1 (RPS6KA1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. PTPRN2

    Plasmid
    #38891
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRN2 (PTPRN2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. MIGR1-Meis1a

    Plasmid
    #78148
    Purpose
    retroviral expression of Meis1a and GFP
    Depositor
    Francesco Blasi
    Insert
    Meis1a (Meis1 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  40. pBABEpuro-CRAF_S259A

    Plasmid
    #51127
    Purpose
    mammalian expression of wt CRAF S259A
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    S259A
    Promoter
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51127-plasmid-map-sequence-id-77385

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Showing: 4241 - 4280 of 8321 results