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We narrowed to 18,759 results for: nls

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Showing: 4241 - 4280 of 18759 results

  15. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

    Plasmid
    #101177
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101177-plasmid-map-sequence-id-194000

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  16. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

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  18. pLenti-D156R LbdCas12a-8xNLS-ALFA

    Plasmid
    #248899
    Purpose
    Lentiviral vector expressing human codon-optimized, ALFA-tagged D156R LbdCas12a (deactivated DNAse domain)
    Depositor
    Michael Aregger
    Insert
    D156R-LbdCas12a-8xNLS-5xALFA-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D832R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248899-plasmid-map-sequence-id-498942

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  19. pLenti-hyper LbdCas12a-8xNLS-ALFA

    Plasmid
    #248900
    Purpose
    Lentiviral vector expressing human codon-optimized, ALFA-tagged hyper LbdCas12a (deactivated DNAse domain)
    Depositor
    Michael Aregger
    Insert
    hyper-LbdCas12a-8xNLS-5xALFA-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D235R/E292R/D350R/D832R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248900-plasmid-map-sequence-id-498943

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  20. pLenti-D156R LbdCas12a-8xNLS-ZIM3

    Plasmid
    #248902
    Purpose
    Lentiviral vector expressing human codon-optimized D156R LbdCas12a (deactivated DNAse domain) fused to ZIM3 KRAB domain
    Depositor
    Michael Aregger
    Insert
    D156R-LbdCas12a-8xNLS-ZIM3-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D832R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248902-plasmid-map-sequence-id-498939

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  21. pLenti-hyper LbdCas12a-8xNLS-ZIM3

    Plasmid
    #248903
    Purpose
    Lentiviral vector expressing human codon-optimized hyper LbdCas12a (deactivated DNAse domain) fused to ZIM3 KRAB domain
    Depositor
    Michael Aregger
    Insert
    hyper-LbdCas12a-8xNLS-ZIM3-Myc-NeoR
    Use
    CRISPR and Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    D156R/D235R/E292R/D350R/D832R
    Promoter
    eIFa
    Available since
    Oct. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248903-plasmid-map-sequence-id-498944

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  22. pTL54_pyCAG-s36GFPmNG-t2A-mCherry3NLS-polyA

    Plasmid
    #223546
    Purpose
    Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CAG, no insulation or mammalian selection gene
    Depositor
    Sally Lowell
    Publication
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-mNeonGreen, mCherry-3NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223546-plasmid-map-sequence-id-442169

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  33. pKSB- PUb-NLSmTurqSV40 GGGG AAGA

    Plasmid
    #174535
    Purpose
    PUb-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    PUb-mTurquoise BsaI cassette
    Expression
    Insect
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174535-plasmid-map-sequence-id-343229

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  37. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107303
    Purpose
    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS2
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107303-plasmid-map-sequence-id-204639

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  38. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107304
    Purpose
    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS3
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107304-plasmid-map-sequence-id-204643

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Showing: 4241 - 4280 of 18759 results