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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Budding Yeast, Herpes Simplex Virus protein VP16, Human)

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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b2AR->M71-IRES-tauGFP-ACNF TV

Plasmid

#105198

Purpose

targeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

b2AR->M71-IRES-tauGFP-ACNF (Adrb2, Olfr151 Mouse)

Use

Mouse Targeting

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594V

Plasmid

#131715

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594V

Promoter

CMV

Available Since

May 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET29-hMIGA2(306-593)-6xhis

Plasmid

#192837

Purpose

Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

6xhis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600K

Plasmid

#131720

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600K

Promoter

CMV

Available Since

Dec. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-D594E

Plasmid

#131714

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594E

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Socs2-SH2

Plasmid

#46505

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs2 (SOCS2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 28-162

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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AL05_pDonor.DMD

Plasmid

#100284

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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AC62_pDonor.DMD.TS.DR

Plasmid

#100288

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pNIC28-Ub

Plasmid

#253392

Purpose

Expression in E. coli of ubiquitin.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG NEDD8

Plasmid

#250151

Purpose

Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG NEDD8

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K69R_K709R POLH (4KR)

Plasmid

#246408

Purpose

Expresses K682R_K686R_K694R_K709R POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

K682R, K686R, K694R, K709R

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682A_K686A_K694A_K709A (4KA) POLH_deltaGG Ubiquitin

Plasmid

#246409

Purpose

Expresses GFP-tagged K682A_K686A_K694A_K709A POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG Ubiquitin

Plasmid

#246410

Purpose

Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG ubiquitin

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_ WT POLH_deltaGG Ubiquitin

Plasmid

#246411

Purpose

Expresses HA-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

HA and ΔGG ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLH_deltaGG NEDD8

Plasmid

#246412

Purpose

Expresses HA-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

HA and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_NLS~WT POLH

Plasmid

#246405

Purpose

Expresses WT POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS~FLAG

Expression

Mammalian

Mutation

The coding sequence for WT POLH has been optimise…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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