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PX458_SIX4_iso1_1

Plasmid

#128247

Purpose

Encodes gRNA for 3' target of human SIX4_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SIX4_iso1 gRNA

Use

CRISPR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF326_iso1

Plasmid

#128236

Purpose

Donor vector for 3' FLAG tag of human ZNF326_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF326_iso1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZBTB49_iso1

Plasmid

#128240

Purpose

Donor vector for 3' FLAG tag of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pIAGO-Hap2

Plasmid

#114460

Purpose

Expression of hybrid AmphDI-PerDI (Hap) glycosyltansferase and perosamine synthase in streptomycetes

Depositor

Patrick Caffrey

Article

Hutchinson et al Chem Biol. 2010 Feb 26;17(2):174-82. doi

Insert

Hap2 + perDII

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMPO 51

Plasmid

#68358

Purpose

Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication origin

Depositor

Eduardo Santero

Article

Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pHES942

Plasmid

#90408

Purpose

Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-SIC1

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L

Plasmid

#27000

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-mitoDsRed

Plasmid

#44386

Purpose

3rd generation lentiviral vector

Depositor

Pantelis Tsoulfas

Article

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoDsRed

Use

Lentiviral

Tags

61 aa targeting sequence of the P1 isoform F1F0-A…

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2

Plasmid

#192782

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet

Plasmid

#138086

Purpose

Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJSK664

Plasmid

#205130

Purpose

Expression of GFP-2xPX (from SnxA) in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-NCOA4

Plasmid

#192806

Purpose

Stable expression of HaloTag7-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pME_G_E_004_UAV_mScarlet-I

Plasmid

#235974

Purpose

Universal acceptor vector with mScarlet-I placeholder for lvl0 assembly

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

UAV_mScar

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry

Plasmid

#180646

Purpose

Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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