We narrowed to 1,249 results for: vent

1. pSEVA63_sgRNA-B0_M13ps

   Plasmid

   #231421

   Purpose

   M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-B0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA47_dCas9-GFP_NOT_iC

   Plasmid

   #231418

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

   Plasmid

   #233241

   Purpose

   Parallel coiled-coil with (GGS)12 linker to prevent condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)12-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA19_sgRNA-C1_M13ps

   Plasmid

   #231411

   Purpose

   M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEVA19_sgRNA-C0_M13ps

   Plasmid

   #231410

   Purpose

   M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KG#244

   Plasmid

   #110927

   Purpose

   Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

   Depositor

   Kenneth Miller

   Article

   Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

   Inserts

   unc-129 promoter
   unc-54 3' control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWM_12x601_25bpLinker

   Plasmid

   #157788

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_25bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCiPS-NIFV

   Plasmid

   #110807

   Purpose

   Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

   Depositor

   David Russell

   Article

   Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

   Insert

   Pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 mutations in the DDE catalyic core motif of the…

   Promoter

   CMV

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. plk1 WT GFP-myc

    Plasmid

    #132963

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge