We narrowed to 13,583 results for: TIM

1. pcDNA3.2/pr-Membrane

   Plasmid

   #120817

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   pr-Membrane ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2/EV

   Plasmid

   #120833

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

   Plasmid

   #120555

   Purpose

   Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   Cas9, NES, ERT2 and GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Orange Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71346

   Purpose

   Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pet28a-His6-Halo-Tev-Keap1

   Plasmid

   #62456

   Purpose

   His6-halo tagged human Keap1 protein for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LENC

   Plasmid

   #111163

   Purpose

   miR-E (miR-30 variant)-based RNAi with mCherry reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.2/NS5

    Plasmid

    #120825

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS5 ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGF-mSox2-ONL

    Plasmid

    #65719

    Purpose

    mSox2 reporter plasmid (expresses ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Sox2 promoter and ONL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pN006

    Plasmid

    #188470

    Purpose

    Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    AsCas12a
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFPV25.1_rpsM_mCerulean3

    Plasmid

    #124904

    Purpose

    rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    rpsM promoter and mCerulean3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Twitch-4 pcDNA3

    Plasmid

    #49533

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-4
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.2/NS2A

    Plasmid

    #120818

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS2A ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIVT/Smarca4

    Plasmid

    #122146

    Purpose

    For making Smarca4 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarca4 (Smarca4 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV500 A-C/EBP

    Plasmid

    #33352

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    C/EBP (Cebpa Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Use

    Tags

    Antares

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRS-LacI-GFP

    Plasmid

    #220198

    Purpose

    mini-Tn7 GFP expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    LacI/pLac

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pN256

    Plasmid

    #188472

    Purpose

    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    eGFP-WPRE-DAISY-4T
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.2/NS4A

    Plasmid

    #120826

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4A ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GADD45 WT-luc

    Plasmid

    #8356

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter WT (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. XE118 CAMKII-T286D-CS2+

    Plasmid

    #16736

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-T286D (Camk2a Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    threonine 286 mutated to aspartate

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pDONR223-MCU

    Plasmid

    #31726

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    *

    Promoter

    None

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.2/NS1

    Plasmid

    #120824

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS1 ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.2/NS4B

    Plasmid

    #120823

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4B ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS-MXT GIRK2

    Plasmid

    #20675

    Depositor

    Henry Lester

    Article

    Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

    Insert

    Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
    

    Use

    Xenopus expression

    Tags

    3' UTR Xenopus B- globin and 5' UTR Xen…

    Expression

    Mutation

    A369V

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge