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MTK4b_001

Plasmid

#123840

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFH71

Plasmid

#128214

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8a_004

Plasmid

#123888

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8_004

Plasmid

#123884

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_001

Plasmid

#123890

Purpose

Encodes the 5' PiggyBac transposon recognition site cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5'PB

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_001

Plasmid

#123870

Purpose

Encodes the 3' PiggyBac transposon recognition site cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3'PB

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSB4C5_J23101-CocE

Plasmid

#128129

Purpose

Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

CocE

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_016

Plasmid

#123731

Purpose

Encodes Gs-coupled rM3D as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

rM3D(Gs)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0446

Plasmid

#124022

Purpose

BxBI Destination vector with H2B::GFP expressed from CAG promoter

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BXBI_pCAG-H2B::msfGFP-bghpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2

Plasmid

#133002

Purpose

pFE carrying both the dabA2 (Uniprot: D0KWS7) and dabB2 genes (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-min2

Plasmid

#126956

Purpose

"Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 L171C

Plasmid

#66177

Purpose

Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Promoter

cpc operon

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Display-5

Plasmid

#54802

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG-Display

Expression

Mammalian

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
SRS-MTBD(26/19)ux, dimer, no myc

Plasmid

#22382

Depositor

Ron Vale

Article

Carter et al Science. 2008 Dec 12. 322(5908):1691-5.

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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YCplac111-KOG1 (pAN58)

Plasmid

#39209

Depositor

Michael Hall

Article

Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

Insert

KOG1 (KOG1 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

L1512P

Promoter

KOG1

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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XE95 mFZ6-CS2+

Plasmid

#16802

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ6-CS2+ (Fzd6 Mouse)

Use

Xenopus expression

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
XE105 XPKC alpha-myc-CS2+

Plasmid

#16745

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

XPKC-alpha-myc (prkca Frog)

Use

Xenopus expression

Tags

c-myc

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pLEW100v5-NEO-T7-sgRNA

Plasmid

#245118

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pTipi2.2a

Plasmid

#252899

Purpose

Golden gate destination vector, allows for expression of recombinant proteins, N and C-term tags

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pTipi2.2b

Plasmid

#252900

Purpose

Golden gate destination vector, allows for expression of recombinant proteins, no N-term tag

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pTipi2.2c

Plasmid

#252901

Purpose

Golden gate destination vector, allows for expression of recombinant proteins, no C-term tag

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pTipi2.2d

Plasmid

#252902

Purpose

Golden gate destination vector, allows for expression of recombinant proteins, no tags

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1_N-NLS

Plasmid

#221867

Purpose

Empty backbone for expressing GFP-fusion proteins in mammalian cells with a nuclear localization signal (NLS).

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Article

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG

Plasmid

#220537

Purpose

AAVS1-targeting backbone used as a filler plasmid to co-deliver during nucleofection with pAAV-Puro_siKD2.0 (Plasmid #220536) cloned with shRNAs

Depositor

Alessandro Bertero

Article

Balmas et al Mol Syst Biol. 2025 Dec 10. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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