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pFPV25.1_dGFPmut3-AAV

Plasmid

#187377

Purpose

rpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Article

Ciolli Mattioli et al Curr Biol. 2023 Nov 20;33(22):4880-4892.

Insert

dGFPmut3_AAV

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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3922-48

Plasmid

#176497

Purpose

pTRE-EGFP-T2A-TFEB(wt)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(wild-type; full-length; human)

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Myc tag S16-A PTB

Plasmid

#23025

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed Serine 16 to Alanine

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
T7-RelA(K221R)

Plasmid

#23248

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K221R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Oct4i

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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NHA-Ago2

Plasmid

#36922

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

Ago2 (AGO2 Human)

Tags

NHA

Expression

Mammalian

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1

Plasmid

#58415

Purpose

Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-kinase30

Plasmid

#59124

Purpose

FAK fret control sensor without FERM domain with 30 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N19_trigger

Plasmid

#132747

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N19_trigger

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K218R)

Plasmid

#23247

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA(K218R) (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K218R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
NpT7-5-ERK1

Plasmid

#39229

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

ERK1 (MAPK3 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pJW4.16

Plasmid

#62871

Purpose

Bacterial expression of MBP-MBK-2

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2

Tags

MBP

Expression

Bacterial

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag DLC2

Plasmid

#24269

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pKD317.8

Plasmid

#181951

Purpose

IPTG-inducible expression of NarX

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)

Expression

Bacterial

Promoter

NarX-Ptac; lacI-PlacIq

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBAC690-TEF

Plasmid

#160432

Purpose

Expresses eGFP under control of the yeast TEF2 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

S. cerevisiae TEF2 promoter

Expression

Yeast

Promoter

S. cerevisiae TEF2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET14b PHAS-I F113A

Plasmid

#15680

Depositor

John Lawrence

Article

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I F113A (Eif4ebp1 Rat)

Tags

6xHis

Expression

Bacterial

Mutation

F113A mutation.

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadR)-DadR

Plasmid

#191636

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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