We narrowed to 2,463 results for: T2A

1. pFUW-LNI

   Plasmid

   #233205

   Purpose

   Lentiviral dox-inducible expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

   Insert

   mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225703

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K36M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   K36M

   Promoter

   pEF1A

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEA1-Puro-HEK3 CTT ins

   Plasmid

   #171996

   Purpose

   Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

   Plasmid

   #181973

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
   

   Use

   Lentiviral

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

   Plasmid

   #85800

   Purpose

   donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Mutation

   homology arms for knock-in into first intron

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

   Plasmid

   #181972

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
   

   Use

   Lentiviral

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-extGluc-NGFR

   Plasmid

   #104832

   Purpose

   extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   extGLuc
   NGFR
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

   Plasmid

   #225698

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K27M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mCherry

   Expression

   Mammalian

   Mutation

   K27M

   Promoter

   pEF1A

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PEA1-Nuclease-Puro HEK3 CTT ins

   Plasmid

   #171995

   Purpose

   Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-UbC-rtTA-Ngn2_2A_Sox11-PU​RO

    Plasmid

    #226284

    Purpose

    Tetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neurons

    Depositor

    Susannah Sample

    Article

    Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

    Insert

    NGN2:T2A:SOX11 (SOX11, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    NGN2 and SOX11 linked via T2A

    Expression

    Mammalian

    Available Since

    Nov. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP

    Plasmid

    #245377

    Purpose

    Cre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.

    Depositor

    D. James Surmeier

    Article

    Geibl et al Mol Neurodegener. 2024 Oct 8;19(1):69. d

    Insert

    Rpl22l1 (Rpl22l1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG, T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1-GA_AIO-TetOn_ISL1-LHX3_Down-Tandem

    Plasmid

    #241395

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ISL1 and LHX3

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3 Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE; CAG

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Tre-Rac1-P29S (FLARE)

    Plasmid

    #241371

    Purpose

    Dox-inducible expression of Rac1-P29S FLARE sensor

    Depositor

    Gaudenz Danuser

    Article

    Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

    Insert

    Pak1, Rac1 (PAK1, RAC1 Human)
    

    Use

    Lentiviral

    Tags

    YPet (N terminal to Pak1) and mCerulean3 (N termi…

    Mutation

    P29S in Rac1

    Promoter

    TRE

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-2G-CNCB_sgRBBP6-68

    Plasmid

    #237555

    Purpose

    A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Insert

    RBBP6 (RBBP6 Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter and mouse U6 promoter, human U6…

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-TR-TTHA

    Plasmid

    #232479

    Purpose

    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Thermus thermophilus HB8
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CMV-TetO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-LNI-bGH

    Plasmid

    #233203

    Purpose

    AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Lhx3, Isl1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-NIL (with mNgn2x3HA)

    Plasmid

    #233192

    Purpose

    Retroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    mNgn2x3HA-P2A-Isl1-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-NLI (with mNgn2x3HA)

    Plasmid

    #233193

    Purpose

    Retroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    mNgn2x3HA-P2A-mLhx3-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-LIN (with mNgn2x3HA)

    Plasmid

    #233194

    Purpose

    Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    mLhx3-P2A-Isl1-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-ILN (with mNgn2x3HA)

    Plasmid

    #233196

    Purpose

    Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

    Insert

    Isl1-P2A-mLhx3-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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