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We narrowed to 5,211 results for: golden gate

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Showing: 2141 - 2160 of 5211 results

  3. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  9. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Publication
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184887-plasmid-map-sequence-id-437896

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  15. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid
    #247262
    Purpose
    Express ISCro4 recombinase and bridge RNA
    Depositor
    Patrick Hsu
    Publication
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    Expression
    Mammalian
    Mutation
    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…
    Available since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247262-plasmid-map-sequence-id-490480

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  17. pAIP53

    Plasmid
    #258140
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion. No cloning scars
    Depositor
    Christopher Bahl
    Publication
    Fram et al bioRxiv [Preprint]. 2023 May 9:2023.05.0
    Insert
    Smt3 (SMT3 )
    Tags
    HisTag (N terminal on insert)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    258140-plasmid-map-sequence-id-513522

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Showing: 2141 - 2160 of 5211 results