We narrowed to 3,421 results for: mpo

1. pcDNA3-AktAR

   Plasmid

   #61624

   Purpose

   FRET-based biosensor for monitoring Akt activity

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR (AKT1 Human, Synthetic, Budding Yeast)
   

   Use

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. WDR5-HaloTag

   Plasmid

   #200881

   Purpose

   Mammalian expression of human WDR5 with C-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   WDR5-HaloTag (WDR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-mCherry-C1-hCRACR2A-isoform-a

   Plasmid

   #79593

   Purpose

   Transient expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

   Insert

   CRACR2A-isoform-a (CRACR2A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-EFS-DIO-GRAB_OTmut

   Plasmid

   #185389

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKII-soCoChR-GFP

   Plasmid

   #107711

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-IFT27

   Plasmid

   #218722

   Purpose

   Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT27 (IFT27 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-Aion-ts-citrine-ER

   Plasmid

   #192575

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   Aion
   

   Use

   AAV

   Tags

   ts-Citrine-ER

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUPI hT5-S322-Cyp

   Plasmid

   #80282

   Purpose

   Expresses human TRIM5 CypA fusion

   Depositor

   Jeremy Luban

   Article

   Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

   Insert

   TRIM5 CypA fusion (TRIM5, PPIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-Cep123 (CCDC123/Cep89)

    Plasmid

    #67787

    Purpose

    This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

    Insert

    Cep123 (CEP89 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. anxA10-GFP

    Plasmid

    #107199

    Purpose

    Expresses human annexin A10-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

    Insert

    annexin A10 (ANXA10 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK314

    Plasmid

    #110545

    Purpose

    Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    Modified PrhaBAD (CRP-binding sites removed)
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PDZb-beta2(616-951)-mCherry

    Plasmid

    #100736

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    PDZb and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. FastLightR-bRaf-mVenus

    Plasmid

    #162155

    Purpose

    Expresses FastLightR-bRaf-mVenus in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    FastLightR-bRaf-mVenus
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Valine 600 is substituted with Glutamic acid

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid

    #86212

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta-Globin (HBB, Hbb Human, Mouse)
    

    Use

    Ponasterone a inducible promoter

    Tags

    lacZA or lacZB

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Ponasterone A inducible

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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