We narrowed to 34,848 results for: CaS;

1. pMCSG7-dNmeCas9

   Plasmid

   #71475

   Purpose

   bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-Inactive-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   D16A H588A

   Promoter

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBZCas13b-HEPN

   Plasmid

   #89901

   Purpose

   Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R116A/H121A/R1177A/H1182A

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CMV-TO-Casp3te

   Plasmid

   #116055

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   Casp3te
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ284 (Mb2Cas12a)

   Plasmid

   #138116

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   Mb2Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PRE-Hsp70BbCas9_1.2

   Plasmid

   #190796

   Purpose

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pTetQCas-8+IS186

   Plasmid

   #170636

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9 iFE

    Plasmid

    #190138

    Purpose

    Expresses SpCas9-POLB (iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-POLB (HiFi iFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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