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VQR variant

Plasmid

#138560

Purpose

Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
GPI-mGreenLantern

Plasmid

#197916

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 9

Plasmid

#8476

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
pFBP-4_1m.mutant

Plasmid

#162869

Purpose

Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1m_RNA-device

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCre-AntiDsRed

Plasmid

#139674

Purpose

Cassette for testing Cre recombinase activity using a fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Cre recombinase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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QQR1 variant

Plasmid

#138564

Purpose

Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

QQR1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HA Raptor mutant 7

Plasmid

#8475

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

738SLQN741-PAAA (S738P, L739A, Q740A, N741A)

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pTRETightBI-RY-HMGA2mutant

Plasmid

#31470

Depositor

Phil Sharp

Article

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Insert

eYFP and mCherry

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only