Addgene

We narrowed to 1,461 results for: p53

Showing: 501 - 550 of 1461 results

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pegRNA_GBA-4

Plasmid

#199274

Purpose

pegRNA used to correct GBA (c. 1226 A > G) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA 1226GtoA pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-CDC25A

Plasmid

#216511

Purpose

Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CDC25A (CDC25A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pegRNA-GBA-10

Plasmid

#199271

Purpose

pegRNA used to induce GBA (c. 1226 A > G) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA 1226AtoG pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYESir2-puro

Plasmid

#1769

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-IREShygro2

Plasmid

#153966

Purpose

Expresses EGFP in mammalian cells

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

EGFP

Use

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CMV promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
shLuc pRRL

Plasmid

#19125

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mLPx-shZMPSTE24

Plasmid

#69067

Purpose

encodes a shRNA against the protease ZMPSTE24

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

shRNA against ZMPSTE24 (ZMPSTE24 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-mdm2/2702

Plasmid

#11492

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pSLIK_hygro_BirA

Plasmid

#136537

Purpose

Lentiviral expression vector for an inducible BirA

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-miR-29b-1~29a

Plasmid

#51376

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

pri-miR-29b-1~29a (MIR29B1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pegRNA_LMNA_CR-2-5

Plasmid

#199275

Purpose

pegRNA used to correct LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824TtoC pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pegRNA_LMNA-1

Plasmid

#199273

Purpose

pegRNA used to induce LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824CtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiGuide_GFP_NT135

Plasmid

#183118

Purpose

pLentiGuide modified to express GFP and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MYB-3'UTR

Plasmid

#25798

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

3'UTR of human MYB (MYB Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Lsg-LRRK2-1

Plasmid

#199277

Purpose

nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 nick

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pegRNA_LRRK2-3

Plasmid

#199272

Purpose

pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 6055GtoA pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ps226

Plasmid

#133557

Purpose

RNAi construct for knock-down of CDKN1A (Cip1, p21)

Depositor

David Livingston

Article

Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

Plasmid

#136544

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Use

Tags

ANXA2 (29-339) and His-tag

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
human pirh2-(1-261)

Plasmid

#24878

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

Plasmid

#136546

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Use

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-p52-HDM2_sensor

Plasmid

#127184

Purpose

Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2

Use

Tags

HisTag and StrepTag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Neo-mdm2C438L/2854

Plasmid

#11496

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

C438L

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pLentiGuide_mCherry_sgEPAS1#2

Plasmid

#183123

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-mdm2C464A/2855

Plasmid

#11493

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C464A

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2_BirA

Plasmid

#136521

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

R118G (highly promiscuous form)

Promoter

Available since

June 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Neo-mdm2H452A/2858

Plasmid

#11497

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

H452A

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-mdm2C438L/2857

Plasmid

#11494

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C438L

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2711

Plasmid

#175083

Purpose

Mammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutations

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Use

Tags

Expression

Mammalian

Mutation

E223A, S226A, RC mutations

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2712

Plasmid

#175084

Purpose

Mammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutations

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)

Use

Tags

Expression

Mammalian

Mutation

E223A, S226A, RN2 mutations

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRevTre-mKLF5

Plasmid

#41744

Depositor

Jonathan Katz

Article

Yang et al Cell Cycle. 2012 Nov 1;11(21):4033-9. do

Insert

Klf5 (Klf5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
GST-ZNF668

Plasmid

#45042

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 (ZNF668 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRC2_3xflag_Luciferase

Plasmid

#136519

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

Luciferase

Use

Entry vector for gateway cloning

Tags

3x FLAG

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
MDM2p-Mdm2-YFP

Plasmid

#53962

Purpose

Expresses YFP tagged Mdm2 from MDM2 promoter

Depositor

Uri Alon, Galit Lahav

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

MDM2 (MDM2 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

MDM2

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
IFI16-FL-(1-729)

Plasmid

#35051

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16 (IFI16 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
VCP F12.4 gRNA

Plasmid

#90939

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F12.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
VCP F11.4 gRNA

Plasmid

#90938

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F11.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
IFI16-FL (pcDNA3-FLAG)

Plasmid

#35064

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16 (IFI16 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
SETD2-GFP

Plasmid

#80653

Purpose

mammalian expression of SETD2-GFP

Depositor

Sérgio De Almeida

Article

Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

Insert

SETD2 (SETD2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BRCA2 A12.2 gRNA

Plasmid

#90551

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A12.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-EGFP-MnSOD

Plasmid

#16612

Depositor

Bert Vogelstein

Article

Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Insert

MnSOD (SOD2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1V5-His Snk/Plk2 T239A

Plasmid

#22219

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Use

Tags

Expression

Mammalian

Mutation

single amino acid substitution mutant, threonine …

Promoter

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
MASTL B4.4 gRNA

Plasmid

#90755

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation B4.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-cyclin E

Plasmid

#216508

Purpose

Expresses cyclin E1 in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CCNE1 (CCNE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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