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pHH0103_MPP4-1/1-IS1/3

Plasmid

#91267

Purpose

Protein expression and purification of human SH3 domain construct MPP4-1/1-IS1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP4-1/1-IS1/3 (MPP4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBS cScx 3'UTR (CT#485)

Plasmid

#13957

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (SCX Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCDF-casBCDE(-3)

Plasmid

#89729

Purpose

Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.

Depositor

Konstantin Kuznedelov, Konstantin Severinov

Article

Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Derivative CRISPR array containing a shorter vers…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS2/3

Plasmid

#91318

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS2/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS3/3

Plasmid

#91560

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS3/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS1/3

Plasmid

#91429

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS1/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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G273D HA-dynamin 1 pUHD10-3

Plasmid

#34689

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1 Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

G273D

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
wt HA-dynamin 1 pUHD10-3

Plasmid

#34688

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1L Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pB[U6-3 wex2 PUb-DsRed]

Plasmid

#169010

Purpose

For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

white ex2 sgRNA

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster U6:3

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

Plasmid

#172985

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-AP-4 (Myc)3-σ4

Plasmid

#198331

Purpose

Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Use

Tags

triple Myc tag

Expression

Mammalian

Mutation

silent substitution in codon 131

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-HA-mAVO3 (pRL62-3)

Plasmid

#39210

Depositor

Michael Hall

Article

Jacinto et al Nat Cell Biol. 2004 Nov;6(11):1122-8. Ep

Insert

mAVO3 (Rictor Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G5S and I15V

Promoter

CMV

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pET21b-Caspase-3(D3A: D175A D28A D9A)

Plasmid

#90090

Purpose

Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

Depositor

Clay Clark

Article

Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-ChR2-mCherry-Fishell-3

Plasmid

#83898

Purpose

ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

ChR2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pBetI-BCD7-phlF-ssrA P69U18C51T15 v40-3 (570)

Plasmid

#70731

Purpose

plasmid for building 3n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

Plasmid

#84256

Purpose

Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

Plasmid

#84253

Purpose

Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV, lentiviral LTR

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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