We narrowed to 18,548 results for: Mut

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pST_pvLEA22mer_mutant_23 (pBS0719)

Plasmid

#185223

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_23. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_23

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_22 (pBS0718)

Plasmid

#185222

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_22. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_22

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_21 (pBS0717)

Plasmid

#185221

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_21. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_21

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_2 (pBS0715)

Plasmid

#185220

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_2

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_17 (pBS0712)

Plasmid

#185219

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_17. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_17

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_16 (pBS0711)

Plasmid

#185218

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_16. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_16

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_14 (pBS0709)

Plasmid

#185217

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_14. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_14

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_10 (pBS0705)

Plasmid

#185216

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSZPKIamutHAERT(#119)

Plasmid

#184076

Purpose

cyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Insert

ZfmutPKAinhiba-HA-ERT2

Use

Synthetic Biology

Tags

internal tag: HA

Expression

Mammalian

Mutation

ZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…

Promoter

sCMV+SP6

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5mutCBS

Plasmid

#131835

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Expression

Mammalian

Mutation

partial intron 4 full exon 5 and partial intron 5…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Expression

Yeast

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2EFMUT

Plasmid

#174333

Purpose

encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

NKD2132DFD134>AFA (substitutions of D132 and D…

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAmut

Plasmid

#153548

Purpose

Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

a 3-bp mutation in exon 6

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Triple mutant

Plasmid

#63719

Purpose

Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Triple mutant

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut3

Plasmid

#53742

Purpose

promoter region of PIM2 lacking the 2 forkhead binding sites

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Mutation

deletion of 2 forkhead binding sites (bp# 395-497…

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut2

Plasmid

#51850

Purpose

promoter region of PIM2 lacking the forkhead binding site #1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
mutpRL-TK 6xUTR

Plasmid

#40761

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
mutpRL-TK 2xUTR

Plasmid

#40757

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

2 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
mutpRL-TK 4xUTR

Plasmid

#40759

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

4 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
mutpRL-TK 5xUTR

Plasmid

#40760

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

5 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only