We narrowed to 17,819 results for: Mut
-
Plasmid#185218PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_16. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertpvLEA22mer_mutant_16
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_pvLEA22mer_mutant_14 (pBS0709)
Plasmid#185217PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_14. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertpvLEA22mer_mutant_14
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_pvLEA22mer_mutant_10 (pBS0705)
Plasmid#185216PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertpvLEA22mer_mutant_10
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSZPKIamutHAERT(#119)
Plasmid#184076Purposecyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsertZfmutPKAinhiba-HA-ERT2
UseSynthetic BiologyTagsinternal tag: HAExpressionMammalianMutationZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…PromotersCMV+SP6Available SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-CD45i4e5i5mutCBS
Plasmid#131835PurposeA reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.DepositorInsertPTPRC (PTPRC Human)
UseTagsExpressionMammalianMutationpartial intron 4 full exon 5 and partial intron 5…PromoterAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBP-2_6m.mutant
Plasmid#162842PurposeYeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6DepositorInsert2_6m_RNA-device
UseTagsExpressionYeastMutationPromoterAvailable SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2EFMUT
Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAmut
Plasmid#153548PurposeUsed as a donor vector for the targeted knock-in of a mutation in PIGA exon 6DepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
UseDonor plasmidTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Triple mutant
Plasmid#63719PurposeVoltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227DDepositorInsertTriple mutant
UseTagsSuper ecliptic pHlorin A227DExpressionMammalianMutationPromoterCMVAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut3
Plasmid#53742Purposepromoter region of PIM2 lacking the 2 forkhead binding sitesDepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationdeletion of 2 forkhead binding sites (bp# 395-497…PromoterAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut2
Plasmid#51850Purposepromoter region of PIM2 lacking the forkhead binding site #1DepositorAvailable SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 6xUTR
Plasmid#40761PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert6 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 2xUTR
Plasmid#40757PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert2 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 4xUTR
Plasmid#40759PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert4 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 5xUTR
Plasmid#40760PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert5 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1xUTR
Plasmid#40756PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-GW1D1aMut(W>A)
Plasmid#21522DepositorInsertTNRC6A (TNRC6A Human)
UseTagsGFPExpressionMammalianMutationcontain aa254-503, mutate every tryptophan (total…PromoterAvailable SinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x mut A
Plasmid#11314DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucExpressionMutationMutation A (see paper) on inner 2 binding sites.PromoterAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x mut B
Plasmid#11315DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucExpressionMutationMutation B (see paper) on inner 2 binding sites.PromoterAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x mut C
Plasmid#11316DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucExpressionMutationMutation C (see paper) on inner 2 binding sites.PromoterAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only