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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-TurboID-NS

Plasmid

#166566

Purpose

Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1

Plasmid

#114022

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTalpha1-Dre

Plasmid

#133925

Purpose

Neuron-specific expression of Dre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

HA-Dre

Tags

HA

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP29

Plasmid

#22599

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP29 (USP29 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1

Plasmid

#114027

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

Plasmid

#244218

Purpose

Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

Plasmid

#244219

Purpose

Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Expression

Mammalian

Available Since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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469_pME_iCre

Plasmid

#171792

Purpose

pME-Vector with codon improved iCre sequence. Tol2-kit compatible.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

codon-optimized iCre sequence

Promoter

None

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Multisite gateway

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Ruby2-NS

Plasmid

#166569

Purpose

Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Ruby2

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LV-Floxed Luc

Plasmid

#60622

Purpose

Expresses a conditional luciferase cassette dependent on Cre mediated recombination

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Floxed Luciferase

Use

Cre/Lox, Lentiviral, and Luciferase

Promoter

human UbC

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP2-NS

Plasmid

#80502

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

TagBFP2 (no STOP)

Use

Multisite gateway middle entry clone

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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1455_pDEST_miniTol2_R4-R2_MCS

Plasmid

#171794

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r237

Plasmid

#133417

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 237

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r237 (Vmn1r237 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-NS

Plasmid

#108885

Purpose

Multisite gateway vector for 5' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r78

Plasmid

#133413

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 78

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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