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pcDNA3-MAVS-M1A,M142A(KaganF62)

Plasmid

#52002

Purpose

Expresses the human MAVS CDS containing two point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-5'UTR-MAVS-ORF1(KaganE49)

Plasmid

#52006

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF1mut

Use

Tags

Expression

Mammalian

Mutation

mutation removing the start codon for ORF1

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204343

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-nLuc

Plasmid

#199756

Purpose

Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Amyloid β42-c_myc

Plasmid

#225223

Purpose

Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

Amyloid β42
Aga2 (AGA2 Budding Yeast)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

Plasmid

#225228

Purpose

Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (25Q)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

Plasmid

#225229

Purpose

Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (46Q)

Use

Tags

HA Tag and c-myc Tag

Expression

Yeast

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKSB- attP 3xP3-GFPsv40 GGGG AAGA

Plasmid

#174534

Purpose

attP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

Depositor

Eric Marois

Insert

attP and 3xP3-GFP-SV40 BsaI cassette

Use

Tags

Expression

Insect

Mutation

Promoter

3xP3

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)

Plasmid

#52018

Purpose

Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pTwist-SARS-CoV-2 Spike-deltaC18 T478R (AGA)

Plasmid

#212533

Purpose

Encodes SARS-CoV-2 spike T478R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T478R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

T478R

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T19R (AGA)

Plasmid

#212417

Purpose

Encodes SARS-CoV-2 spike T19R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

T19R

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Plasmid

#199759

Purpose

Elongation reporter construct to quantify elongation duration of 4_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Plasmid

#199760

Purpose

Elongation reporter construct to quantify elongation duration of 6_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Use

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T_CIP2A_1-560_R522D

Plasmid

#217923

Purpose

E. coli expression of GST-tagged CIP2A_1-560 with R522D mutation

Depositor

Jukka Westermarck

Insert

CIP2A (CIP2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

R522D (522 AGA->GAT)

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5 DPC4 (1-514)

Plasmid

#14040

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (1-514) (SMAD4 Human)

Use

Tags

Expression

Mammalian

Mutation

R515 was mutated into a stop codon. AGA to TGA. D…

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only