We narrowed to 2,316 results for: pET28

1. pET28a-MSP1D1delltaH4

   Plasmid

   #71713

   Purpose

   Membrane scaffold protein (MSP), helix 4 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-DDX43

   Plasmid

   #134574

   Purpose

   Express human DDX43 protein (WT, full-length) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43 (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length WT

   Promoter

   T7

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-5'TwStrep

   Plasmid

   #208620

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28 Set9 wt

   Plasmid

   #24082

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-PFO

   Plasmid

   #92109

   Purpose

   Recombinant expression in E.Coli

   Depositor

   Feng Shao

   Article

   Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

   Insert

   PFO
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Delta27aa

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_T5-ARG1

   Plasmid

   #106476

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_Caspase-4_p20_C258A

   Plasmid

   #214307

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   C258A

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28HistevTtTERT(1-516)

    Plasmid

    #28171

    Depositor

    Kathleen Collins

    Article

    O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

    Insert

    Tetrahymena TERT
    

    Use

    Tags

    6xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-PHGDH

    Plasmid

    #74113

    Purpose

    Expression of N-terminal His-tagged

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Tags

    His-tagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-T4uvsW

    Plasmid

    #228201

    Purpose

    Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

    Depositor

    Stephen Benkovic

    Article

    Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

    Insert

    uvsW
    

    Use

    Tags

    hexahistidine and 10 amino acid linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-RILP

    Plasmid

    #74600

    Purpose

    used for expression in E. coli and purification

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab-interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-NM23-H1

    Plasmid

    #157987

    Purpose

    Expresses NM23-H1 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

    Insert

    NM23-H1 (NME1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge