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We narrowed to 1,175 results for: poli

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

Plasmid

#46838

Purpose

Express cGFP transcript ending in the minimal functional MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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PABPC1_544-626_pGEX6P1

Plasmid

#209790

Purpose

Bacterial expression of PABPC1 Mlle domain

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

Insert

Mlle domain of PABPC1 (PABPC1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 544-626 only

Promoter

tac

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-cGAS-HA

Plasmid

#231895

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Use

Tags

3xHA and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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emiRFP670-cGAS

Plasmid

#231896

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P-2-human RNF146 full length

Plasmid

#132610

Purpose

expresses human RNF146 full length in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 (RNF146 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-MGMT

Plasmid

#187654

Purpose

Expresses hMGMT together with the puromycin resistance gene via an IRES.

Depositor

Robert Sobol

Article

Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

Insert

MGMT (MGMT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPTG-GG-sg2+sg3

Plasmid

#127199

Purpose

Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

tRNA-gRNA

Use

Empty sgrna plasmid

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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Ubc-c005

Plasmid

#216876

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c004

Plasmid

#216875

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-eRF3a F76a

Plasmid

#65811

Purpose

expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

eRF3a (GSPT1 Human)

Use

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

F76a

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAG32-3HA-3FLAG

Plasmid

#154081

Purpose

For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

Depositor

Cornelia Kilchert

Article

Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

Insert

3xHA-3xFLAG-hygR

Use

Pcr-based tagging in yeast

Tags

3xHA-3xFLAG

Expression

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-SRO1 JN177

Plasmid

#192546

Purpose

pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 SR3

Plasmid

#192547

Purpose

pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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