We narrowed to 2,322 results for: puc19

1. pUC19-link-PAGFP-FLAG:Kan

   Plasmid

   #211834

   Purpose

   Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAGFP-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-TSV (linearized in VP2)

   Plasmid

   #70032

   Purpose

   pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

   Depositor

   Mariet Feltkamp

   Article

   van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

   Insert

   TSV full genome, linearized in VP2 gene
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-Top-Toehold-mCherry

   Plasmid

   #87318

   Purpose

   Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19 ACMV DNA-B 1.5mer

   Plasmid

   #159135

   Purpose

   Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

   Depositor

   Vincent Fondong

   Article

   Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

   Insert

   ACMV DNA-B 1.5mer
   

   Use

   Infectious clone for bombarding plants

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-P70a-Top-T~Bottom-T

   Plasmid

   #87315

   Purpose

   Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system (promoter control)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 gene 60 delta gap

   Plasmid

   #49091

   Purpose

   Template for generating gene 60 mRNA, coding gap deletion

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Use

   Tags

   Expression

   Mutation

   Coding gap and coding gap STOP have been deleted.

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mishler2014-No-aptamer-controlluciferase-pUC19

   Plasmid

   #63856

   Purpose

   This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.

   Depositor

   Howard Salis

   Article

   Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

   Insert

   No-aptamer control mRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tac

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pUC19::ZmUBI-RWT4Ca_K217R/K327R

   Plasmid

   #237511

   Purpose

   Protoplast expression

   Depositor

   Gitta Coaker

   Article

   Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

   Insert

   Rwt4Ca_K217R/K327R
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutations at kinase active sites (K217R, K327R)

   Promoter

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19_msfGFP-HP1a-repair-template

    Plasmid

    #238248

    Purpose

    For knock-in msfGFP to HP1a locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    HP1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19_msfGFP-TCOF1-repair-template

    Plasmid

    #237632

    Purpose

    For knock-in msfGFP to TCOF1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19_msfGFP-FUS-repair-tempate

    Plasmid

    #237684

    Purpose

    For knock-in msfGFP to FUS locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    FUS (FUS Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19::ZmUBI-RWT4Ca+C-Tail

    Plasmid

    #237514

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca+C-Tail
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Add C-terminal extension

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-Bs3-sgRNA (BsaI)

    Plasmid

    #188280

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

    Insert

    Bs3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    U6

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19 KGA HA5'-mKO2-3'

    Plasmid

    #110406

    Purpose

    Homology donor for glutaminase KGA isoform knock-in, mKO2

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. puC19 preACT1(del6) short BP-3'SS

    Plasmid

    #111333

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    shorter branch to 3' splice site distance

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. puC19 preACT1(del6) AcAc

    Plasmid

    #111332

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    3' splice site AcAc

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pre-Ubc4 BrG pUC19

    Plasmid

    #111322

    Purpose

    for transcription to Ubc4 pre-mRNA

    Depositor

    Christine Guthrie

    Article

    Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

    Insert

    UBC4 (UBC4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    intron has branch-site A to G mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19 gene 60 thru gap

    Plasmid

    #49160

    Purpose

    Template for generating gene 60 mRNA truncated two codons after coding gap

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Use

    Tags

    Expression

    Mutation

    gene 60 mRNA truncated two codons after coding ga…

    Promoter

    T7

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19 gene 60 to gap + Cys

    Plasmid

    #49159

    Purpose

    Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 600
    

    Use

    Tags

    Expression

    Mutation

    Coding gap and downstream sequence have been dele…

    Promoter

    T7

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge