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Plasmid#158633PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro
Plasmid#158639PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ148A/Q271APromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Ala148 hygro
Plasmid#158636PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-MAVS-Gln93 hygro
Plasmid#158635PurposeLentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant constructDepositorInsertMAVS (MAVS Human)
UseLentiviralTags3XFLAGExpressionMutationE93QPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-EFa1-HA-Jag1
Plasmid#46051DepositorInsertJag1 (Jag1 Rat)
UseTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_ZIKV_NS5_MTase
Plasmid#213529PurposeExpresses the MTase domain of NS5 of ZIKVDepositorInsertZIKA virus NS5 methyltransferase domain
UseTagsHis6-sumoExpressionBacterialMutationMTase domain aa 1-265PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-YapS127A
Plasmid#46049DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsFlag tagExpressionMammalianMutationS127APromoterEF1aAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP5G
Plasmid#31788Purposea single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.DepositorInsertGCaMP5G
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET-RCaMP1h
Plasmid#42874Purposea single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRubyDepositorInsertRed Fluorescent Calcium binding Protein
UseTagsEK (Enterokinase) Recognition Site, His Tag (6x),…ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN1 Lck-GCaMP5G
Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorInsertLck-GCaMP5G
UseTagsExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-Yap S127A
Plasmid#46050DepositorInsertYes-kinase associated protein (YAP1 Human)
UseEntry vectorTags2X Flag tagExpressionMutationS127APromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A
Plasmid#213527PurposeExpresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92ADepositorInsertNSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationD90A and E92APromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_hRNMT_RAM
Plasmid#213528PurposeExpresses the human RNMT-RAM complexDepositorInsertsUseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14
Plasmid#213518PurposeExpresses NSP14 of SARS-CoV-2DepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsN-terminal His6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_OC43_NSP14
Plasmid#213519PurposeExpresses NSP14 of hCoV OC43DepositorInserthCoV OC43 NSP14 (ORF1ab hCoV OC43)
UseTagsHis-SUMO tagExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_NL63_NSP14
Plasmid#213520PurposeExpresses NSP14 of hCoV NL63DepositorInserthCoV NL63 NSP14 (ORF1ab hCoV NL63)
UseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_229E_NSP14
Plasmid#213521PurposeExpresses NSP14 of hCoV 229EDepositorInserthCoV 229E NSP14
UseTagsHis6-sumoExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_V290A
Plasmid#213522PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation V290ADepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
UseTagsHis6-sumoExpressionBacterialMutationchanged V290 to APromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only