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Showing: 3271 - 3300 of 8804 results

  1. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

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  3. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  10. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid
    #196415
    Purpose
    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smMyc
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196415-plasmid-map-sequence-id-385068

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  17. AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

    Plasmid
    #196414
    Purpose
    Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smFLAG
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196414-plasmid-map-sequence-id-385111

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  18. pCX-SpiCee-Kras

    Plasmid
    #140838
    Purpose
    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Insert
    SpiCee-Kras
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140838-plasmid-map-sequence-id-304816

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  20. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Use
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  22. AAV-GfaABC1D-lck-smV5-4x6T-WPRE

    Plasmid
    #196416
    Purpose
    Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smV5
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196416-plasmid-map-sequence-id-385161

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  23. CANE-LV envelope

    Plasmid
    #86666
    Purpose
    pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus
    Depositor
    Fan Wang
    Article
    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:
    Insert
    CANE-LV envelope
    Use
    Lentiviral, Mouse Targeting, and Synthetic Biolog…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  27. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172473-plasmid-map-sequence-id-342817

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  29. pRosa26-TG

    Plasmid
    #40026
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    T (ATG)
    beta-globin intron
    Neo
    GFP
    diphteria toxin A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides 1-274 and insertion of FRT, …
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    40026-plasmid-map-sequence-id-210931

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Showing: 3271 - 3300 of 8804 results